Department of Microbiology and Immunology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
J Glob Antimicrob Resist. 2022 Sep;30:414-417. doi: 10.1016/j.jgar.2022.08.001. Epub 2022 Aug 6.
Co-transfer of carbapenem and amikacin resistance might contribute to the evolution of extensively drug resistant (XDR) Acinetobacter baumannii. The current study used bioinformatic tools and an in silico approach to investigate the potential mobility of a novel composite transposon co-harboring bla and aphA6.
The transposon, named here Tn7382, was recently identified in the chromosomes of two XDR A. baumannii isolates (M02 and M11) from Egypt. The draft genomes of M02 and M11 were generated by Illumina sequencing. Nucleotide homology of Tn7382 and flanking regions was analyzed using the Basic Local Alignment Search Tool.
Tn7382 is derived from Tn125 and encompasses seven open reading frames [aphA6, ISAba125 transposase-coding gene, bla, ble, iso, tat, cutA] enclosed by two direct copies of ISAba14. While described for the first time, Tn7382 was found in the chromosomes of five A. baumannii strains deposited in the NCBI database. Using the Artemis Comparison Tool, the potential mobility of Tn7382 was demonstrated in silico by comparative genomic analysis of two A. baumannii strains (TP1 and TP2) retrieved from the NCBI database. The transposon was acquired by TP2 at the same location as an ISAba14 element in the ancestral variant TP1 isolated from the same patient in the USA 11 days earlier.
Here, we present the characteristics of Tn7382, a composite transposon flanked by ISAba14 and harboring the aphA6 and bla resistance genes. In silico analysis inferred the potential mobility of Tn7382, but experimental validation is still required.
碳青霉烯类和阿米卡星耐药性的共同转移可能导致广泛耐药（XDR）鲍曼不动杆菌的进化。本研究使用生物信息学工具和计算机模拟方法研究了一种新型复合转座子共同携带 bla 和 aphA6 的潜在可移动性。
该转座子名为 Tn7382，最近在埃及分离的两株 XDR 鲍曼不动杆菌（M02 和 M11）的染色体中被发现。M02 和 M11 的草图基因组通过 Illumina 测序生成。使用 Basic Local Alignment Search Tool 分析 Tn7382 和侧翼区域的核苷酸同源性。
Tn7382 源自 Tn125，包含七个开放阅读框[aphA6、ISAba125 转座酶编码基因、bla、ble、iso、tat、cutA]，由两个 ISAba14 的直接拷贝包围。虽然这是首次描述，但 Tn7382 已在 NCBI 数据库中存储的五株鲍曼不动杆菌菌株的染色体中被发现。使用 Artemis Comparison Tool，通过比较基因组分析从 NCBI 数据库中检索到的两株鲍曼不动杆菌（TP1 和 TP2），在计算机模拟中证明了 Tn7382 的潜在可移动性。该转座子是由 TP2 在与从同一患者在美国 11 天前分离的原始变体 TP1 中的 ISAba14 元件相同的位置获得的。
在这里，我们介绍了 Tn7382 的特征，这是一个由 ISAba14 侧翼的复合转座子，携带 aphA6 和 bla 耐药基因。计算机模拟分析推断了 Tn7382 的潜在可移动性，但仍需要实验验证。
