Kumar Vikas, Swain Himanshu Sekhar, Roy Sankhajit, Das Basanta Kumar, Upadhyay Aurobinda, Ramteke Mitesh Hiradas, Kumar Vikash, Kole Ramen Kumar, Banerjee Hemanta
Department of Agricultural Chemicals, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia 741252, West Bengal, India; ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata 700120, West Bengal, India.
ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata 700120, West Bengal, India.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Nov;261:109427. doi: 10.1016/j.cbpc.2022.109427. Epub 2022 Aug 6.
Butachlor herbicide belongs to the family of chloroacetanilide group, widely used for control of grass and broadleaf weeds in paddy fields however, its repeated application may result in aquatic pollution. Butachlor residue has been detected in aquatic environments, which may produce toxic effects on non-target organisms including fish. Keeping this in mind, the present study was designed to estimate the LC of butachlor (Shaktiman®), and to evaluate the sub-lethal toxicity at two concentrations (12.42 μg L and 62.10 μg L) in Labeo rohita for a period of 24, 48, and 72 h. Fish exposed to butachlor reduced the counts of red blood cells (RBC), haemoglobin (HGB), hematocrit (HCT), and white blood cells (WBC). A significant (p < 0.05) increase in the antioxidant enzyme (superoxide dismutase-SOD, glutathione-s-transferase-GST), and hepatic enzyme (glutamate-oxaloacetate transaminase-GOT, glutamate-pyruvate transaminase-GPT) were noticed in butachlor exposed fish. Heat shock protein 70 (HSP70) and HSP90 in gill; cortisol, protein, albumin, globulin, and triglyceride in serum were increased upon exposure of butachlor. On the contrary, complement 3 (C3) and immunoglobulin (IgM) in serum was found to be decreased compared to control fish. The findings thus suggest that the fish upon exposure to butachlor disrupts the biomarkers which ultimately leads to growth retardation in fish.
丁草胺除草剂属于氯代乙酰苯胺类，广泛用于稻田防除禾本科和阔叶杂草。然而，其重复施用可能导致水体污染。在水生环境中已检测到丁草胺残留，这可能对包括鱼类在内的非目标生物产生毒性作用。考虑到这一点，本研究旨在估计丁草胺（Shaktiman®）的半数致死浓度，并评估在两种浓度（12.42μg/L和62.10μg/L）下对露斯塔野鲮进行24、48和72小时的亚致死毒性。暴露于丁草胺的鱼类红细胞（RBC）、血红蛋白（HGB）、血细胞比容（HCT）和白细胞（WBC）数量减少。在暴露于丁草胺的鱼类中，抗氧化酶（超氧化物歧化酶-SOD、谷胱甘肽-s-转移酶-GST）和肝酶（谷氨酸草酰乙酸转氨酶-GOT、谷氨酸丙酮酸转氨酶-GPT）显著（p<0.05）增加。丁草胺暴露后，鳃中的热休克蛋白70（HSP70）和HSP90；血清中的皮质醇、蛋白质、白蛋白、球蛋白和甘油三酯增加。相反，与对照鱼相比，血清中的补体3（C3）和免疫球蛋白（IgM）减少。因此，研究结果表明，鱼类暴露于丁草胺会破坏生物标志物，最终导致鱼类生长迟缓。
