Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea; Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, Republic of Korea.
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Bioresour Technol. 2021 Mar;324:124674. doi: 10.1016/j.biortech.2021.124674. Epub 2021 Jan 7.
In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01. Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved. Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%). Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides. Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications. EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.
在本研究中，从韩国东海（束草海滩）分离出一株产胞外多糖（EPS）的细菌菌株，并鉴定为黄杆菌属 Yanoikuyae BBL01。通过响应面设计进行了培养基优化，得到了 2.63±0.02 g/L EPS 的产量。使用乳糖作为主要碳源生产的纯化 EPS 通过 GC-MS 分析，由 α-D-木吡喃糖（28.6±2.0%）、β-D-吡喃葡萄糖（21.0±1.6%）、α-D-吡喃甘露糖（18.5±1.2%）、β-D-吡喃甘露糖（13.1±1.4%）、β-D-木吡喃糖（10.2±2.1%）、α-D-吡喃半乳糖（5.9±1.1%）和β-D-半乳糖醛酸（2.43±0.8%）组成。有趣的是，不同的碳源（葡萄糖、半乳糖、甘油、乳糖、蔗糖和木糖）对 EPS 单体组成没有影响，各种单糖的质量百分比略有变化。纯化的 EPS 稳定高达 233°C，表明其可能适合用作食品相关应用的增稠和胶凝剂。EPS 还表现出相当的乳化、絮凝、自由基清除和金属络合活性，表明具有各种生物技术应用。
