痘苗病毒晚期信使核糖核酸的不连续转录或核糖核酸加工会产生一个5'多聚腺苷酸前导序列。
Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5' poly(A) leader.
作者信息
Schwer B, Visca P, Vos J C, Stunnenberg H G
出版信息
Cell. 1987 Jul 17;50(2):163-9. doi: 10.1016/0092-8674(87)90212-1.
Abstract
We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5' terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position -1, confirms the existence of a poly(A) leader of approximately 35 A-residues.
摘要
我们通过引物延伸和帽分析证明，成熟痘苗病毒晚期转录本是不连续合成的。我们已经表明，来自易位的11K以及真实的11K和4b晚期启动子的RNA转录本，比使用痘苗基因组DNA作为探针通过S1图谱确定的“起始位点”延伸了约35个核苷酸。RNA和第一链cDNA的测序表明，一个同聚的聚（A）序列与RNA转录本的5'末端相连。用含有A链的DNA探针（取代-1位置上游的启动子序列）对RNA转录本进行S1图谱分析，证实了存在约35个A残基的聚（A）前导序列。
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