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痘苗病毒指导合成含有5'多聚腺苷酸序列的早期信使核糖核酸。

Vaccinia virus directs the synthesis of early mRNAs containing 5' poly(A) sequences.

作者信息

Ink B S, Pickup D J

机构信息

Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.

出版信息

Proc Natl Acad Sci U S A. 1990 Feb;87(4):1536-40. doi: 10.1073/pnas.87.4.1536.

DOI:10.1073/pnas.87.4.1536
PMID:1968253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC53510/
Abstract

mRNAs transcribed from late promoters of several poxvirus genes contain 5' poly(A) sequences that are not complementary to the viral DNA. In contrast, early mRNAs containing 5' poly(A) sequences have not previously been identified. Modifications to the sequence of the promoter of an early gene of cowpox virus enable this promoter to direct the synthesis of RNAs containing 5' poly(A) sequences. When the sequence 3'-ATTTA-5', which is present at the RNA start-sites of several late promoters, is positioned such that the RNA start-site of the early promoter is at the first thymidylate in this sequence, this early promoter directs the synthesis of early RNAs containing 4-11 adenylates at their 5' ends. When two of the thymidylates in the sequence 3'-ATTTA-5' are removed, the promoter directs the synthesis of early RNAs lacking 5' poly(A) sequences. These results are consistent with the proposal that 5' polyadenylylation of poxvirus RNAs occurs by repetitive transcription of thymidylates in the sequence 3'-ATTTA-5' often present at the sites of transcriptional initiation. In addition, these results demonstrate that 5' polyadenylylation of viral RNAs is not exclusively a late function. The promoter regions of a few early genes of vaccinia virus contain the sequence 3'-ATTTA-5'. Analyses of the transcripts of one of these genes, the D5 gene, indicated that these mRNAs contain 5' poly(A) sequences, suggesting that early mRNAs of a subset of viral genes contain 5' poly(A) sequences.

摘要

从几种痘病毒基因的晚期启动子转录而来的mRNA含有5'多聚腺苷酸序列，这些序列与病毒DNA不互补。相比之下，此前尚未鉴定出含有5'多聚腺苷酸序列的早期mRNA。对牛痘病毒一个早期基因启动子序列的修饰，使该启动子能够指导合成含有5'多聚腺苷酸序列的RNA。当存在于几个晚期启动子RNA起始位点的3'-ATTTA-5'序列的位置，使得早期启动子的RNA起始位点位于该序列的第一个胸苷酸处时，这个早期启动子指导合成在其5'端含有4-11个腺苷酸的早期RNA。当3'-ATTTA-5'序列中的两个胸苷酸被去除时，该启动子指导合成缺乏5'多聚腺苷酸序列的早期RNA。这些结果与痘病毒RNA的5'多聚腺苷酸化是通过在转录起始位点常见的3'-ATTTA-5'序列中胸苷酸的重复转录发生的这一观点一致。此外，这些结果表明病毒RNA的5'多聚腺苷酸化并非仅仅是晚期功能。痘苗病毒一些早期基因的启动子区域含有3'-ATTTA-5'序列。对其中一个基因D5基因转录本的分析表明，这些mRNA含有5'多聚腺苷酸序列，这表明病毒基因子集的早期mRNA含有5'多聚腺苷酸序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/29d665ae5b83/pnas01029-0289-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/347ac14947c0/pnas01029-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/64caad2568c0/pnas01029-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/a8f3249910c8/pnas01029-0288-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/eaaf562d258b/pnas01029-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/5033ceaa184e/pnas01029-0289-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/29d665ae5b83/pnas01029-0289-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/347ac14947c0/pnas01029-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/64caad2568c0/pnas01029-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/a8f3249910c8/pnas01029-0288-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/eaaf562d258b/pnas01029-0289-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/5033ceaa184e/pnas01029-0289-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6ea/53510/29d665ae5b83/pnas01029-0289-c.jpg

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