Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, Balanagar, Telangana, 500037, India.
Bioanalysis. 2022 Jun;14(12):881-909. doi: 10.4155/bio-2022-0095. Epub 2022 Aug 10.
Green bioanalytical techniques aim to reduce or eliminate the hazardous waste produced by bioanalytical technologies. A well-organized and practical approach towards bioanalytical method development has an enormous contribution to the green analysis. The selection of the appropriate sample extraction process, organic mobile phase components and separation technique makes the bioanalytical method green. UHPLC-MS is the best option, whereas supercritical fluid chromatography is one of the most effective green bioanalytical procedures. Nevertheless, there remains excellent scope for further research on green bioanalytical methods. This review details the various sample preparation techniques that follow green analytical chemistry principles. Furthermore, it presents green solvents as a replacement for conventional organic solvents and highlights the strategies to convert modern analytical techniques to green methods.
绿色生物分析技术旨在减少或消除生物分析技术产生的有害废物。对于绿色分析而言，朝着有组织和实用的生物分析方法开发方向发展具有巨大的贡献。选择适当的样品提取过程、有机流动相成分和分离技术可使生物分析方法更加绿色环保。UHPLC-MS 是最佳选择，而超临界流体色谱则是最有效的绿色生物分析程序之一。然而，在绿色生物分析方法方面仍有很大的研究空间。本文详细介绍了遵循绿色分析化学原理的各种样品制备技术。此外，还介绍了将绿色溶剂替代传统有机溶剂的方法，并强调了将现代分析技术转化为绿色方法的策略。
