Faculty of Biomedical Sciences, Department of Microbiology and Immunology, Kampala International University, Ishaka, Bushenyi, Uganda.
Analytical Biochemistry Research Centre, Universiti Sains Malaysia, Bayan Lepas, Pulau Pinang, Malaysia.
PLoS One. 2022 Aug 10;17(8):e0272799. doi: 10.1371/journal.pone.0272799. eCollection 2022.
Horseshoe crabs are one of the most studied invertebrates due to their remarkable innate immunity mechanism and biological processes. In this work, the proteins of the lipopolysaccharides (LPS)-stimulated and non-stimulated hemocytes of Malaysian Tachypleus gigas were profiled using LC-MS/MS. A total of 154 proteins were identified in both types of samples. Additionally, seventy-seven proteins were commonly found in both conditions, while 52 and 25 proteins were uniquely found in the LPS-stimulated and non-stimulated hemocytes, respectively. ATP-dependent energy-generating proteins such as actins and BLTX actin-related proteins were detected in both stimulated and non-stimulated T. gigas hemocytes, but more of such proteins were found in the former type. Proteins such as tachylectin-2, coagulogen, c-reactive proteins, histones, hemocyanin, and DNA polymerase, which play key roles in the organism's innate immunity, were differentially expressed in the hemocytes following LPS challenge. In conclusion, the proteins identified in the hemolymph of T. gigas are vital for the organism's molecular functions, biological processes, and activation of innate immunity.
马蹄蟹因其显著的先天免疫机制和生物学过程而成为研究最多的无脊椎动物之一。在这项工作中，使用 LC-MS/MS 对马来西亚 Tachypleus gigas 的脂多糖 (LPS) 刺激和非刺激血细胞中的蛋白质进行了分析。在这两种类型的样本中，共鉴定出 154 种蛋白质。此外，77 种蛋白质在两种条件下均被发现，而 52 种和 25 种蛋白质分别在 LPS 刺激和非刺激血细胞中特异性发现。在 LPS 刺激和非刺激的 T. gigas 血细胞中都检测到了肌动蛋白和 BLTX 肌动蛋白相关蛋白等依赖于 ATP 的能量生成蛋白，但前者中发现的此类蛋白更多。在 LPS 刺激后，在血细胞中差异表达了参与机体先天免疫的关键作用的蛋白，如 tachylectin-2、凝血酶原、C 反应蛋白、组蛋白、血蓝蛋白和 DNA 聚合酶。总之，在 T. gigas 血淋巴中鉴定出的蛋白质对机体的分子功能、生物学过程和先天免疫的激活至关重要。
