Department of Beverage Research, Chair Analysis and Technology of Plant-Based Foods, Geisenheim University, Von-Lade-Straße 1, D-65366 Geisenheim, Germany.
Department of Enology, Chair Wine and Beverage Chemistry, Geisenheim University, Von-Lade-Straße 1, D-65366 Geisenheim, Germany.
J Agric Food Chem. 2022 Aug 24;70(33):10349-10358. doi: 10.1021/acs.jafc.2c02782. Epub 2022 Aug 10.
Potassium dihydrogen phosphonate had been allowed as a plant strengthener in organic viticulture in the European Union only until 2013, supporting the control of grapevine downy mildew. Therefore, low or nondetectable levels are a prerequisite for marketing of organic wines and, consequently, validated analytical methods are of major interest. Herein, two methods based on ion chromatography conductivity detection (IC-CD) or ion chromatography inductively coupled plasma mass spectrometry (IC-ICP-MS) for the determination of phosphonic acid (HPO) from 14 different plant matrices of L., wine, and soil were developed, validated, and compared. Extraction recoveries ranged from 95.1 to 99.3%. Limits of quantification (LOQ) ranged in liquid and solid samples from 3.8 to 16.8 μg/kg and 0.08 to 2.41 mg/kg for ICP-MS detection and from 39.9 to 593.7 μg/kg and 3.51 to 58.7 mg/kg for CD, respectively. Data on a current anonymized selection of 100 conventionally and 30 organically produced wines are briefly presented to demonstrate the suitability of the method.
磷酸二氢二氢磷曾被允许在欧盟的有机葡萄种植中作为植物强化剂使用，直到 2013 年，用于控制葡萄霜霉病。因此，低水平或无法检测到的水平是有机葡萄酒销售的前提条件，因此，经过验证的分析方法具有重要意义。在此，开发并比较了两种基于离子色谱电导检测（IC-CD）或离子色谱电感耦合等离子体质谱（IC-ICP-MS）的方法，用于测定 14 种不同植物基质（L.、葡萄酒和土壤）中的磷酸（HPO）。提取回收率范围为 95.1%至 99.3%。对于 ICP-MS 检测，液体和固体样品的定量限（LOQ）范围分别为 3.8 至 16.8 μg/kg 和 0.08 至 2.41 mg/kg，对于 CD 分别为 39.9 至 593.7 μg/kg 和 3.51 至 58.7 mg/kg。简要介绍了当前对 100 种常规和 30 种有机生产葡萄酒的匿名选择数据，以证明该方法的适用性。
