University of Calabar, Calabar.
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Niger J Physiol Sci. 2022 Jun 30;37(1):49-58. doi: 10.54548/njps.v37i1.7.
Impaired male reproductive function is a major complication associated with diabetes mellitus (DM). Whether or not insulin, when co-administered with zinc will reverse or ameliorate reproductive dysfunction in male diabetics is not known. This study thus sought to establish if co-administration of insulin and zinc reverses or ameliorates male reproductive dysfunction in DM better than either insulin or zinc. Five (5) normal and twenty (20) diabetic sexually mature rats were assigned into five groups of five animals each. Group A consisted of normal rats and had access to only food and water. Group B consisted of diabetic animals with no treatment and served as DM control. Groups C and D consisted of diabetic animals and received insulin and zinc respectively. Group E consisted of diabetic animals and received both insulin and zinc. All diabetic animals had free access to food and water. Insulin in all cases was given subcutaneously twice daily in the morning and evening at 1 unit and 4 units respectively. Zinc (10mg/kg) was given orally once daily. Treatments in all cases commenced two weeks after DM was confirmed. The treatment lasted ten days. Samples were thereafter collected for analyses. DM decreased sperm count, sperm motility, sperm viability, normal sperm cells, semen pH, serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone, while increasing sperm cells with defective tails. DM also impaired testicular morphology. Insulin and zinc co-administration improved sperm viability, sertoli cell count, Johnsen's score, serum FSH, LH and testosterone. Co-administration also improved semen pH towards normal. Insulin or zinc ameliorated several aspects of DM-induced male sexual dysfunction. However, the co-administration of insulin and zinc provided better results.
男性生殖功能障碍是糖尿病（DM）的主要并发症之一。胰岛素与锌联合使用是否可以逆转或改善男性糖尿病患者的生殖功能障碍尚不清楚。因此，本研究旨在确定胰岛素和锌联合使用是否比单独使用胰岛素或锌更能逆转或改善 DM 患者的男性生殖功能障碍。将五（5）只正常和二十（20）只糖尿病性成熟大鼠分为五组，每组五只动物。A 组由正常大鼠组成，仅可获得食物和水。B 组由未经治疗的糖尿病动物组成，作为 DM 对照组。C 组和 D 组由糖尿病动物组成，分别接受胰岛素和锌治疗。E 组由糖尿病动物组成，同时接受胰岛素和锌治疗。所有糖尿病动物都可以自由获得食物和水。所有情况下，胰岛素均每日两次皮下注射，早晚分别为 1 单位和 4 单位。锌（10mg/kg）每日口服一次。所有情况下的治疗均在确认 DM 后两周开始，持续十天。然后收集样本进行分析。DM 降低了精子计数、精子活力、精子活力、正常精子细胞、精液 pH 值、血清卵泡刺激素（FSH）、黄体生成素（LH）和睾酮，同时增加了尾部有缺陷的精子细胞。DM 还损害了睾丸形态。胰岛素和锌联合使用可提高精子活力、支持细胞计数、Johnsen 评分、血清 FSH、LH 和睾酮。联合使用还使精液 pH 值向正常方向改善。胰岛素或锌可改善 DM 引起的男性性功能障碍的几个方面。然而，胰岛素和锌的联合使用提供了更好的结果。
