Parvin Shahram, Shahriary Alireza, Aghamollaei Hossein, Gh B Fatemeh Nobakht M, Bagheri Hasan, Ghanei Mostafa, Daryabari Seyed-Hashem, Jadidi Khosrow, Arabfard Masoud
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Education Office, Pasture Institute of Iran, Tehran, Iran.
Proteome Sci. 2022 Aug 10;20(1):13. doi: 10.1186/s12953-022-00195-1.
Understanding the molecular and cellular mechanisms involved in the pathogenesis of ocular injured induced by mustard gas can help better identify complications and discover appropriate therapies. This study aimed to analyze the proteomics of tears of chemical warfare victims with mustard gas ocular injuries and compare it with healthy individuals. In this case-control research, 10 mustard gas victims with long-term ocular difficulties (Chronic) were included in the patient group, while 10 healthy persons who were age and sex matched to the patients were included in the control group. Schirmer strips were used to collect the tears of the participants. Proteomics experiments were performed using the high-efficiency TMT10X method to evaluate the tear protein profile, and statistical bioinformatics methods were used to identify the differently expressed proteins. 24 proteins had different expressions between the two groups. Among these 24 proteins, 8 proteins had increased expression in veterans' tears, while the remaining 16 proteins had decreased expression. Reactome pathways were used to look at proteins with various expressions, and 13 proteins were found to be engaged in the immune system, 9 of which were effective in the innate immune system, and 5 proteins were effective in the complement cascade. Ocular mustard gas exposure may cause a compromised immune system on the eye's surface, exposing the cornea to external and endogenous infections, and eventually causing corneal opacity and reduced vision.
了解芥子气所致眼损伤发病机制中涉及的分子和细胞机制，有助于更好地识别并发症并发现合适的治疗方法。本研究旨在分析芥子气眼损伤化学战受害者泪液的蛋白质组学，并与健康个体进行比较。在这项病例对照研究中，患者组纳入了10名有长期眼部问题（慢性）的芥子气受害者，而对照组纳入了10名年龄和性别与患者匹配的健康人。使用泪液试纸收集参与者的泪液。采用高效TMT10X方法进行蛋白质组学实验以评估泪液蛋白质谱，并使用统计生物信息学方法鉴定差异表达的蛋白质。两组之间有24种蛋白质存在差异表达。在这24种蛋白质中，8种蛋白质在退伍军人泪液中的表达增加，而其余16种蛋白质的表达减少。使用Reactome通路研究差异表达的蛋白质，发现13种蛋白质参与免疫系统，其中9种在先天免疫系统中起作用，5种蛋白质在补体级联反应中起作用。眼部接触芥子气可能会导致眼表免疫系统受损，使角膜暴露于外部和内源性感染，最终导致角膜混浊和视力下降。
