Ren Zhouzheng, Yan Jiakun, Whelan Rose, Liao Xujie, Bütz Daniel E, Arendt Maria K, Cook Mark E, Yang Xiaojun, Crenshaw Thomas D
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Department of Animal Sciences, University of Wisconsin, Madison, WI, USA.
Anim Nutr. 2022 Jun 22;10:382-389. doi: 10.1016/j.aninu.2022.06.008. eCollection 2022 Sep.
Oral antibody to interleukin-10 (anti-IL-10) enhances the intestinal immune defense against . The sulfur amino acids methionine and cysteine (M+C) play essential roles in inducing and maintaining protective immune responses during intestinal infections. Hence, increased dietary M+C may support the anti-IL-10-induced intestinal immunity to . Broilers ( = 640) were arranged in a 2 × 2 × 2 factorial design with 2 levels of each of the 3 main factors: dietary standardized ileal digestible (SID) M+C levels (0.6% or 0.8%), dietary anti-IL-10 supplementation (with or without), and coccidiosis challenge (control or challenge). Briefly, the broilers were supplied with either 0.6% or 0.8% SID M+C, each with or without anti-IL-10 (300 μg/kg), from d 10 to 21. On d 14, broilers from each diet were gavaged with either PBS or . The resulting infection induced fecal oocyst shedding and intestinal lesions. Broilers fed 0.8% SID M+C (main effects, ≤ 0.05) had decreased feed-to-gain ratio, increased duodenum and cecum luminal anti IgA titers, and decreased fecal oocyst counts, when compared to 0.6% SID M+C. The supplementation of anti-IL-10 (main effects, ≤ 0.05) increased cecum luminal total IgA concentration and decreased cecum lesions. Interactions ( ≤ 0.05) were detected for growth performance and cecum luminal IFN-γ. Briefly, the highest body weight gain and feed intake were reached in PBS-gavaged broilers fed 0.8% SID M+C with no anti-IL-10 and in -challenged broilers fed 0.8% SID M+C with anti-IL-10. In -infected broilers, anti-IL-10 increased intestinal luminal IFN-γ and body weight gain only at 0.8% SID M+C. Collectively, anti-IL-10 increased intestinal luminal IFN-γ levels, decreased cecum lesions and restored growth only when fed with adequate amounts of sulfur amino acids. Our findings underscore the importance of providing sufficient essential nutrients to support the anti-IL-10 induced immunity against coccidiosis.
白细胞介素-10口服抗体（抗IL-10）可增强肠道对……的免疫防御。含硫氨基酸蛋氨酸和半胱氨酸（M+C）在肠道感染期间诱导和维持保护性免疫反应中发挥着重要作用。因此，增加日粮中的M+C可能会增强抗IL-10诱导的肠道对……的免疫力。将640只肉鸡按2×2×2析因设计进行安排，3个主要因素各有2个水平：日粮标准化回肠可消化（SID）M+C水平（0.6%或0.8%）、日粮抗IL-10添加情况（添加或不添加）以及球虫病攻毒（对照或攻毒）。简而言之，从第10天至第21天，给肉鸡提供0.6%或0.8%的SID M+C，每种日粮添加或不添加抗IL-10（300μg/kg）。在第14天，给每种日粮的肉鸡灌胃PBS或……。由此引发的……感染导致粪便卵囊排出和肠道病变。与0.6%的SID M+C相比，饲喂0.8%的SID M+C（主效应，P≤0.05）的肉鸡料重比降低，十二指肠和盲肠腔抗IgA滴度升高，粪便卵囊计数减少。添加抗IL-10（主效应，P≤0.05）可提高盲肠腔总IgA浓度并减轻盲肠病变。在生长性能和盲肠腔IFN-γ方面检测到了交互作用（P≤0.05）。简而言之，在未添加抗IL-10且饲喂0.8%的SID M+C的灌胃PBS的肉鸡以及添加抗IL-10且饲喂0.8%的SID M+C的……攻毒肉鸡中，体重增加和采食量最高。在……感染的肉鸡中，抗IL-10仅在SID M+C为0.8%时才会增加肠道腔IFN-γ水平和体重。总体而言，只有在饲喂足够量含硫氨基酸时，抗IL-10才会增加肠道腔IFN-γ水平、减轻盲肠病变并恢复生长。我们的研究结果强调了提供足够必需营养素以支持抗IL-10诱导的抗球虫病免疫的重要性。
