来自杏仁的Bet v 1同源物Pru du 1，是桦树花粉相关杏仁过敏患者的主要过敏原。

Pru du 1, the Bet 1-homologue from almond, is a major allergen in patients with birch pollen associated almond allergy.

作者信息

Kabasser Stefan, Crvenjak Nadja, Schmalz Stefanie, Kalic Tanja, Hafner Christine, Dubiela Pawel, Kucharczyk Aleksandra, Bazan-Socha Stanislawa, Lukaszyk Mateusz, Breiteneder Heimo, Radauer Christian, Bublin Merima

机构信息

Institute of Pathophysiology and Allergy Research Center of Pathophysiology Infectiology and Immunology, Medical University of Vienna Vienna Austria.

Department of Dermatology University Hospital St. Pölten Karl Landsteiner University of Health Sciences St. Pölten Austria.

出版信息

Clin Transl Allergy. 2022 Aug 8;12(8):e12177. doi: 10.1002/clt2.12177. eCollection 2022 Aug.

DOI:10.1002/clt2.12177

PMID:35949989

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9358727/

Abstract

BACKGROUND

Almond allergy is common and can manifest in two different forms. Primary almond allergy has been reported to be associated with sensitization to almond legumin Pru du 6. In birchendemic regions, there is a link between birch-pollinosis which is likely based on a cross-reactive Bet v 1 homologue, a yet unidentified allergen in almond. Therefore, we sought to identify and characterize a Bet v 1-homologue in almond.

METHODS

The expression of a Bet v 1 homologue in almond kernels was confirmed by mass spectrometry. The recombinant protein was produced in and its cross-reactivity and allergenic potency was analyzed by IgE quantitative and competitive ELISA, immunoblotting and basophil histamine release using sera from 17 almond allergic patients.

RESULTS

The identified Bet v 1 homologue received the designation Pru du 1.0101. Pru du 1.0101 bound IgE from 82 % of almond allergic patients. Bet v 1 was able to inhibit IgE-binding to rPru du 1 by 100%, while rPru du 1 inhibited IgE binding to rBet v 1 by 48%. Pru du 1.0101 activated basophils, though 100- to 1000-fold higher concentrations were required for maximum activation in comparison to rBet v 1.

CONCLUSION

Considering the strong inhibition capacity and higher allergenic potency of Bet v 1, the results provide compelling evidence for primary sensitization to Bet v 1 in case of birch pollen associated almond allergy. Combining Pru du 6 and Pru du 1 in diagnostic approaches may help to discriminate between primary and birch-pollen associated almond allergy.

摘要

背景

杏仁过敏很常见，可表现为两种不同形式。据报道，原发性杏仁过敏与对杏仁豆球蛋白Pru du 6的致敏有关。在桦树流行地区，桦树花粉症之间存在联系，这可能基于一种交叉反应性的Bet v 1同源物，一种尚未确定的杏仁过敏原。因此，我们试图鉴定并表征杏仁中的一种Bet v 1同源物。

方法

通过质谱法证实了杏仁核中Bet v 1同源物的表达。在[具体表达系统未给出]中产生重组蛋白，并使用17名杏仁过敏患者的血清通过IgE定量和竞争性ELISA、免疫印迹和嗜碱性粒细胞组胺释放分析其交叉反应性和致敏效力。

结果

鉴定出的Bet v 1同源物被命名为Pru du 1.0101。Pru du 1.0101与82%的杏仁过敏患者的IgE结合。Bet v 1能够100%抑制IgE与rPru du 1的结合，而rPru du 1抑制IgE与rBet v 1的结合达48%。Pru du 1.0101激活嗜碱性粒细胞，尽管与rBet v 1相比，最大激活需要高100至1000倍的浓度。