Monja-Mio Kelly M, Rescalvo-Morales Antonio, Robert Manuel L
Centro de Investigación Científica de Yucatán, Unidad de Biotecnología, Mérida, Yucatán, Mexico.
Methods Mol Biol. 2022;2527:59-67. doi: 10.1007/978-1-0716-2485-2_5.
Agave fourcroydes (henequén) is a plant used for the extraction of hard fiber from its leaves. Due to its long-life cycle, it is very difficult to genetically improve. Somatic embryogenesis (SE) is a very useful micropropagation technique, that can be used for genetic improvement programs and increase the micropropagation of this species. SE is a morphogenic process by which somatic embryos are generated from somatic cells reprogramming. To initiate the regeneration program, the loss of cell-cell communication is suggested to be important. The Thin Cell Layer (TCL) technique allows for the isolation of specific cell or tissue layers, and in conjunction with strictly controlled growth conditions, may lead to the in vitro induction of specific morphogenic programs. Here, we describe a new protocol for the induction of somatic embryogenesis through TCL culture technique, from stem of elite clonal A. fourcroydes vitroplants previously generated through micropropagation of adventitious shoots.
剑麻（龙舌兰麻）是一种用于从其叶片中提取硬质纤维的植物。由于其生命周期长，很难进行基因改良。体细胞胚胎发生（SE）是一种非常有用的微繁殖技术，可用于基因改良计划并增加该物种的微繁殖。体细胞胚胎发生是一个形态发生过程，通过体细胞重编程产生体细胞胚。为启动再生程序，细胞间通讯的丧失被认为很重要。薄细胞层（TCL）技术允许分离特定的细胞或组织层，并且与严格控制的生长条件相结合，可能会导致体外诱导特定的形态发生程序。在此，我们描述了一种通过TCL培养技术从先前通过不定芽微繁殖产生的优良克隆剑麻离体植株的茎中诱导体细胞胚胎发生的新方案。
