Ramírez-Mosqueda Marco A
Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Amatlán de los Reyes, Veracruz, Mexico.
Methods Mol Biol. 2022;2527:267-270. doi: 10.1007/978-1-0716-2485-2_19.
One of the main objectives to achieve in plant tissue culture is the multiplication of the available plant material, taking full advantage of the regenerative capacities of plant cells. Somatic embryogenesis leverages cell totipotency to produce new explants from a cell, thus obtaining many propagules for scientific research, industrial, or exploitation purposes. Somatic embryogenesis (ES) characterizes by being one of the most efficient techniques in plant micropropagation. However, developing an efficient plant ES protocol requires several key factors to consider, as demonstrated throughout the chapters of this book. These chapters highlight the major drivers of the success of ES in different plant species: plant growth regulators, concentration of auxins and cytokines, water deficit, photoperiod, and type of culture medium; techniques such as the use of bioreactors and Thin Cell Layer (TCL); and the influence of stress on the formation of somatic embryos. Research has been conducted to address each phase of somatic embryogenesis, either individually or for all phases. The chapters of this book cover in detail the techniques used and provide guidance that will allow readers to successfully develop all the somatic embryogenesis phases in different cultures, from cell dedifferentiation to differentiation.
植物组织培养要实现的主要目标之一是充分利用植物细胞的再生能力，增加可用植物材料的数量。体细胞胚胎发生利用细胞全能性从一个细胞产生新的外植体，从而获得许多用于科研、工业或开发目的的繁殖体。体细胞胚胎发生（ES）是植物微繁殖中最有效的技术之一。然而，制定一个高效的植物ES方案需要考虑几个关键因素，本书各章节对此均有阐述。这些章节强调了不同植物物种中ES成功的主要驱动因素：植物生长调节剂、生长素和细胞分裂素的浓度、水分亏缺、光周期和培养基类型；生物反应器和薄细胞层（TCL）等技术；以及胁迫对体细胞胚形成的影响。针对体细胞胚胎发生的每个阶段，无论是单独研究还是对所有阶段进行研究，都开展了相关研究。本书各章节详细介绍了所使用的技术，并提供了指导，使读者能够在不同培养物中成功开展从细胞去分化到分化的所有体细胞胚胎发生阶段。
