Almeida Felipe Astolpho, Santa-Catarina Claudete, Silveira Vanildo
Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil.
Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
Methods Mol Biol. 2022;2527:83-95. doi: 10.1007/978-1-0716-2485-2_7.
Somatic embryogenesis is the process by which embryos are formed from a single or small group of somatic cells in response to specific stimuli. Somatic embryogenesis has been applied to achieve mass clonal propagation on an industrial scale and to increase the agronomic performance of species of economic interest, including sugarcane. The use of somatic embryogenesis in sugarcane stands out as a biotechnological tool with a high potential for application in the clonal propagation of disease-free elite varieties, as an essential part of genetic transformation protocols, and in the production of synthetic seeds. A better understanding of each phase of somatic embryogenesis can help to optimize the process to enhance yields and produce high-quality emblings. In this chapter, we describe a detailed protocol for somatic embryogenesis in sugarcane (Saccharum sp.) to be used in research projects for small-scale production. This protocol comprises all steps from explant preparation to the establishment of sugarcane emblings.
体细胞胚胎发生是指体细胞在特定刺激下由单个或一小群体细胞形成胚胎的过程。体细胞胚胎发生已被应用于实现工业规模的大规模克隆繁殖，并提高包括甘蔗在内的具有经济价值物种的农艺性能。体细胞胚胎发生在甘蔗中的应用作为一种生物技术工具脱颖而出，在无病优良品种的克隆繁殖、基因转化方案的重要组成部分以及合成种子的生产中具有很高的应用潜力。更好地理解体细胞胚胎发生的每个阶段有助于优化该过程，以提高产量并生产高质量的胚状体。在本章中，我们描述了一种用于甘蔗（Saccharum sp.）体细胞胚胎发生的详细方案，该方案可用于小规模生产的研究项目。该方案包括从外植体制备到甘蔗胚状体建立的所有步骤。
