Cava Daniel G, Vélez Marisela
Instituto de Catálisis y Petroleoquímica (CSIC), (Cantoblanco) Madrid, Spain.
Methods Mol Biol. 2022;2538:1-11. doi: 10.1007/978-1-0716-2529-3_1.
Atomic force microscopy (AFM) provides high-resolution images of the topography of amyloid fibers adsorbed on surfaces. This information is very useful to study their molecular assembly under various conditions. This chapter describes the basic protocols required to deposit fibers on flat surfaces and discusses some of the practical issues required to operate a good commercial microscope setup to obtain appropriate high-resolution AFM topographic images of amyloid fibers.
原子力显微镜（AFM）可提供吸附在表面的淀粉样纤维形貌的高分辨率图像。这些信息对于研究它们在各种条件下的分子组装非常有用。本章介绍了将纤维沉积在平面上所需的基本方案，并讨论了操作良好的商用显微镜设置以获得淀粉样纤维合适的高分辨率AFM形貌图像所需的一些实际问题。
