Cava Daniel G, Vélez Marisela
Instituto de Catálisis y Petroleoquímica (CSIC), (Cantoblanco) Madrid, Spain.
Methods Mol Biol. 2022;2538:109-116. doi: 10.1007/978-1-0716-2529-3_8.
Supported lipid bilayers (SLBs) are model membrane systems that can be used to study the interaction between amyloid fibers and membranes with atomic force microscopy (AFM). This chapter describes the preparation of SLBs on mica that can then be used as a substrate for fiber absorption. AFM can then be used to study the topography of the lipid-protein surface to study the evolution of the fibers, as well as the modifications on the membrane induced by their presence.
支持脂质双层（SLBs）是一种模型膜系统，可用于通过原子力显微镜（AFM）研究淀粉样纤维与膜之间的相互作用。本章描述了在云母上制备SLBs的方法，然后可将其用作纤维吸附的底物。接着可以使用AFM研究脂质-蛋白质表面的形貌，以研究纤维的演变以及它们的存在对膜引起的修饰。
