College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266000, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China.
J Agric Food Chem. 2022 Aug 31;70(34):10611-10619. doi: 10.1021/acs.jafc.2c04423. Epub 2022 Aug 11.
Parvalbumin (PV) is the most common allergen in fish. Some patients with fish allergy are allergic to only one species of fish but are tolerant to others; however, the underlying mechanism has not been identified. This study showed that three types of glycated fishes' PV showed a similar decrease in immunoglobulin E (IgE) binding. Glycosylation could improve the simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion resistance of fishes' PV. We also discovered that the cross-reactivity between eel and turbot was weaker than that of bass; glycosylation can reduce cross-reactivity between eel/bass and turbot by downregulating Th2 cytokines and upregulating Th1 cytokines as well as downregulating the expression of G-T PV, G-E PV, G-B PV of IL-4 (94.31 ± 3.16, 73.26 ± 0.91, 94.95 ± 3.03 ng/mL), and IL-13 (38.84 ± 0.75, 33.77 ± 0.71, 36.51 ± 0.50 ng/mL) and upregulating the expression of IFN-γ (318.01 ± 3.46, 387.15 ± 3.30, 318.01 ± 4.21 ng/mL) compared with T PV, respectively. This study showed that glycosylation affected sensitization by regulating the cross-reactivity of parvalbumins.
副肌球蛋白（PV）是鱼类中最常见的过敏原。一些鱼类过敏患者仅对一种鱼类过敏，但对其他鱼类耐受；然而，其潜在机制尚未确定。本研究表明，三种类型的糖化鱼类的 PV 表现出相似的 IgE 结合降低。糖基化可以提高鱼类 PV 在模拟胃液（SGF）和模拟肠液（SIF）中的消化抗性。我们还发现，鳗鱼和大菱鲆之间的交叉反应性比鲈鱼弱；糖基化可以通过下调 Th2 细胞因子和上调 Th1 细胞因子以及下调 IL-4（94.31±3.16、73.26±0.91、94.95±3.03ng/mL）和 IL-13（38.84±0.75、33.77±0.71、36.51±0.50ng/mL）的表达以及上调 IFN-γ（318.01±3.46、387.15±3.30、318.01±4.21ng/mL）的表达来降低鳗鱼/鲈鱼和大菱鲆之间的交叉反应性。本研究表明，糖基化通过调节副肌球蛋白的交叉反应性来影响致敏作用。
