Avalos-Ruiz Diane, Ten Leonid N, Kim Chang-Kil, Lee Seung-Yeol, Jung Hee-Young
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea.
Plant Pathol J. 2022 Aug;38(4):403-409. doi: 10.5423/PPJ.NT.04.2022.0051. Epub 2022 Aug 1.
In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF- 21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA- strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and noshoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.
在诸如镰刀菌属等生物颗粒中，已观察到冰核活性（INA）。利用tef1和rpb1基因通过多位点序列分析对从韩国苹果树衰退病中分离出的镰刀菌菌株进行了鉴定。使用丁香假单胞菌丁香致病变种KACC 21200作为阳性对照，通过液滴冷冻和试管冷冻试验来测定这些菌株的冰核活性，结果得到了7株冰核活性阳性（INA+）的真菌菌株，它们被鉴定为三线镰刀菌（KNUF-21-F17、KNUF-21-F18、KNUF-21-F29、KNUF-21-F32、KNUF-21-F38、KNUF-21-F43和KNUF-21-F44）。将镰刀菌冰核活性阳性菌株KNUF-21-F29与冰核活性阴性（INA-）菌株对菊花品种新玛外植体的影响进行了比较。观察到更高的愈伤组织形成和无芽形成，这表明真菌冰核活性可能在冷害中起作用，并且是苹果快速衰退中需要考虑的一个因素。据我们所知，这是韩国分离出冰核活性真菌菌株的首次报道。
