Insect Biochemistry and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, Raja Rammohunpur, Siliguri, District-Darjeeling, West Bengal, 734013, India.
Sci Rep. 2022 Aug 11;12(1):13653. doi: 10.1038/s41598-022-17739-2.
Dengue is the most rapidly spreading vector-borne disease with an estimated 100-400 million cases each year. Control of Dengue vectors largely depends upon synthetic pyrethroids. Development of insecticide resistance in Aedes mosquitoes however, poses severe threat to insecticide-based vector management programme. Mutations in the Voltage Gated Sodium Channel gene (vgsc) serve as the primary machinery behind this resistance development. In Aedes albopictus, at least four such kdr (knock down resistance) mutations had already been documented. Here, we describe the occurrence of F1534C kdr mutation in wild population of Ae. albopictus from northern part of West Bengal, India including a novel T1520I mutation. Four populations of Ae. albopictus from the studied region were found resistant against DDT and synthetic pyrethroids, among them only one population possessed F1534C kdr mutation. A total of 200 successful amplification followed by partial sequencing of vgsc gene further revealed the presence of F1534C kdr mutation in both phenotypically susceptible and resistant mosquito specimen. Studied populations were found 81% homozygote susceptible (1534F/F), 12.5% heterozygote (1534F/C) and 6% homozygote resistant (1534C/C) for F1534C kdr mutation. The findings of the current study will help to uncover the mechanisms underlying insecticide resistance and hence to reduce errors in vector control measurements.
登革热是传播速度最快的虫媒传染病，每年估计有 1 亿至 4 亿例。登革热媒介的控制主要依赖于合成拟除虫菊酯。然而，埃及伊蚊对杀虫剂的抗药性发展对基于杀虫剂的病媒管理计划构成了严重威胁。电压门控钠离子通道基因（vgsc）的突变是这种抗药性发展的主要机制。在白纹伊蚊中，已经记录了至少四种这样的 kdr（击倒抗性）突变。在这里，我们描述了在印度西孟加拉邦北部的野生白纹伊蚊种群中 F1534C kdr 突变的发生情况，包括一个新的 T1520I 突变。在所研究地区的四个白纹伊蚊种群中，发现它们对滴滴涕和合成拟除虫菊酯具有抗性，其中只有一个种群存在 F1534C kdr 突变。总共对 200 个成功扩增的 vgsc 基因进行部分测序，进一步揭示了在表型敏感和抗性蚊标本中都存在 F1534C kdr 突变。研究人群中，F1534C kdr 突变的纯合敏感型（1534F/F）占 81%，杂合型（1534F/C）占 12.5%，纯合抗性型（1534C/C）占 6%。目前研究的结果将有助于揭示抗药性的机制，从而减少病媒控制测量中的错误。
