多组学分析揭示生菜高温诱导抽薹的分子基础。

Molecular basis of high temperature-induced bolting in lettuce revealed by multi-omics analysis.

机构信息

Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.

College of Horticulture, China Agricultural University, Beijing, 100193, China.

出版信息

BMC Genomics. 2022 Aug 12;23(1):580. doi: 10.1186/s12864-022-08814-z.

DOI:10.1186/s12864-022-08814-z

PMID:35953780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9373282/

Abstract

BACKGROUND

High temperature induces early bolting in lettuce (Lactuca sativa L.), which affects both quality and production. However, the molecular mechanism underlying high temperature-induced bolting is still limited.

RESULTS

We performed systematical analysis of morphology, transcriptome, miRNAs and methylome in lettuce under high temperature treatment. Through a comparison of RNA-Seq data between the control and the high temperature treated lettuces at different time points totally identified 2944 up-regulated genes and 2203 down-regulated genes, which cover three floral pathways including photoperiod, age and gibberellin (GA) pathways. Genome wide analysis of miRNAs and methylome during high temperature treatment indicated miRNAs and DNA methylation might play a role controlling gene expression during high temperature-induced bolting. miRNA targets included some protein kinase family proteins, which potentially play crucial roles in this process.

CONCLUSIONS

Together, our results propose a possible regulation network involved in high temperature-induced bolting.

摘要

背景

高温会导致生菜（Lactuca sativa L.）早期抽薹，这会影响生菜的质量和产量。然而，高温诱导抽薹的分子机制仍很有限。

结果

我们对高温处理下生菜的形态、转录组、miRNA 和甲基组进行了系统分析。通过对不同时间点高温处理和对照生菜的 RNA-Seq 数据进行比较，总共鉴定出 2944 个上调基因和 2203 个下调基因，这些基因涵盖了光周期、年龄和赤霉素（GA）途径三个花发育途径。高温处理过程中 miRNA 和甲基组的全基因组分析表明，miRNA 和 DNA 甲基化可能在高温诱导抽薹过程中控制基因表达。miRNA 的靶标包括一些蛋白激酶家族蛋白，它们可能在这个过程中发挥关键作用。