丘脑室旁核中的谷氨酸能神经元调节异氟醚麻醉的恢复。
Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia.
机构信息
Department of Critical Care Medicine, Affiliated Hospital of Zunyi Medical University, Guizhou Province, 563003, Zunyi city, China.
Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi city, 563003, Guizhou Province, China.
出版信息
BMC Anesthesiol. 2022 Aug 11;22(1):256. doi: 10.1186/s12871-022-01799-y.
OBJECTIVES
Previous studies have demonstrated that the paraventricular nucleus of the thalamus (PVT) is a key wakefulness-controlling nucleus in the thalamus. Therefore, PVT may also be involved in the process of general anesthesia. This study intends to explore the role of PVT in isoflurane anesthesia.
METHODS
In the present study, we used the expression of c-Fos to observe the neuronal activity of PVT neurons under isoflurane anesthesia. We further recorded the effect of isoflurane anesthesia on the calcium signal of PVT glutamatergic neurons in real time with the help of calcium fiber photometry. We finally used chemogenetic technology to specifically regulate PVT glutamatergic neurons, and observed its effect on isoflurane anesthesia and cortical electroencephalography (EEG) in mice.
RESULTS
We found that glutamatergic neurons of PVT exhibited high activity during wakefulness and low activity during isoflurane anesthesia. Activation of PVT glutamatergic neuronal caused an acceleration in emergence from isoflurane anesthesia accompanied with a decrease in EEG delta power (1-4 Hz). Whereas suppression of PVT glutamatergic neurons induced a delay recovery of isoflurane anesthesia, without affecting anesthesia induction.
CONCLUSIONS
Assuming a pharmacokinetic explanation for results can be excluded, these results demonstrate that the PVT is involved in regulating anesthesia emergence.
目的
先前的研究表明，丘脑室旁核（PVT）是丘脑内控制清醒的关键核团。因此，PVT 可能也参与全身麻醉过程。本研究旨在探讨 PVT 在异氟醚麻醉中的作用。
方法
在本研究中，我们使用 c-Fos 的表达来观察 PVT 神经元在异氟醚麻醉下的神经元活动。我们进一步借助钙光纤光度法实时记录异氟醚麻醉对 PVT 谷氨酸能神经元钙信号的影响。最后，我们使用化学遗传技术特异性调节 PVT 谷氨酸能神经元，并观察其对小鼠异氟醚麻醉和皮质脑电图（EEG）的影响。
结果
我们发现，PVT 的谷氨酸能神经元在清醒时表现出高活性，而在异氟醚麻醉时活性降低。PVT 谷氨酸能神经元的激活导致异氟醚麻醉苏醒加速，同时 EEG 德尔塔功率（1-4 Hz）降低。而 PVT 谷氨酸能神经元的抑制则导致异氟醚麻醉恢复延迟，而不影响麻醉诱导。
结论
假设可以排除药代动力学解释的结果，这些结果表明 PVT 参与调节麻醉苏醒。
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