Yan Sha, Sun Minghui, Wang Xuan, Shan Jihao, Xue Xiaofeng
College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China.
Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Foods. 2022 Aug 3;11(15):2316. doi: 10.3390/foods11152316.
The adulteration of honey with different sugar syrups is common and difficult to detect. To ensure fair trade and protect the interests of apiarists, a rapid, simple and cost-effective detection method for adulterants in honey is needed. In this work, fluorescence emission spectra were obtained for honey and sugar syrups between 385 and 800 nm with excitation at 370 nm. We found substantial differences in the emission spectra between five types of honey and five sugar syrups and also found differences in their frequency doubled peak (FDP) intensity at 740 nm. The intensity of the FDP significantly declined (p < 0.01) when spiking honey with ≥10% sugar syrup. To validate this method, we tested 20 adulterant-positive honey samples and successfully identified 15 that were above the limit of detection. We propose that fluorescence spectroscopy could be broadly adopted as a cost-effective, rapid screening tool for sugar syrup adulteration of honey through characterization of emission spectra and the intensity of the FDP.
蜂蜜被不同糖浆掺假的情况很常见且难以检测。为确保公平交易并保护养蜂人的利益，需要一种快速、简单且经济高效的蜂蜜掺假物检测方法。在这项工作中，以370 nm为激发波长，获得了蜂蜜和糖浆在385至800 nm之间的荧光发射光谱。我们发现五种蜂蜜和五种糖浆的发射光谱存在显著差异，并且还发现它们在740 nm处的倍频峰（FDP）强度也有所不同。当向蜂蜜中添加≥10%的糖浆时，FDP的强度显著下降（p < 0.01）。为验证该方法，我们测试了20个掺假阳性蜂蜜样本，并成功鉴定出15个超过检测限的样本。我们建议，通过表征发射光谱和FDP强度，荧光光谱法可作为一种经济高效的快速筛查工具，广泛用于检测蜂蜜中糖浆掺假的情况。
