Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Gora, Prof. Z. Szafrana 1, 65-516 Zielona Gora, Poland.
Food Chem. 2014 Feb 15;145:319-26. doi: 10.1016/j.foodchem.2013.08.069. Epub 2013 Aug 26.
The suitability of a right-angle geometry three-dimensional synchronous fluorescence spectroscopy (3D-SFS) for the differentiation and classification of different honey types, including acacia, buckwheat, heather, linden, multiflower and rape honeys, was evaluated with reference to their phenolic profiles. The fraction of phenolic compounds was initially isolated and pre-concentrated from analyzed samples by means of polymeric reversed phase Strata X solid phase extraction (SPE) cartridges. A thoughtful analysis of 3D-SF spectra of resulting methanolic extracts of honey samples containing the separated fraction of phenolics and standards of phenolic acids and flavonoids revealed that the proposed methodology based on SPE and 3D-SFS is capable of discriminating honey samples due to different floral sources in a fast and straightforward way.
直角几何三维同步荧光光谱法（3D-SFS）对于不同类型蜂蜜（包括刺槐、荞麦、石楠、椴树、百花和油菜蜂蜜）的鉴别和分类的适用性，通过参考其酚类图谱进行了评估。通过聚合反相 Strata X 固相萃取（SPE）小柱，从分析样品中初步分离和预浓缩酚类化合物的分数。对包含分离酚类部分的蜂蜜样品的甲醇提取物和酚酸和类黄酮标准品的 3D-SF 光谱进行了深入分析，结果表明，基于 SPE 和 3D-SFS 的方法能够快速、直接地鉴别由于不同花卉来源而导致的蜂蜜样品。
