Katz R L, Raval P, Manning J T, McLaughlin P, Barlogie B
Diagn Cytopathol. 1987 Jun;3(2):91-101. doi: 10.1002/dc.2840030202.
The biologic and clinical heterogeneity of the various subtypes of non-Hodgkin's lymphoma is related to differences in morphologic, immunologic, and kinetic properties. Comprehensive studies characterizing these features in lymphomatous effusion have yet to be reported. We recently studied 27 effusion specimens from 26 patients with clinically suspected or confirmed lymphoma. Wright-Giemsa- and Papanicolaou-stained cytologic preparations, acridine orange nucleic acid flow cytometry, and immunoperoxidase staining of cell suspensions using antibodies to a battery of T and B cell markers were evaluated and compared with prior histologic accessions. Specimens were classified by cytologic characteristics according to the International Working Formulation Scheme and by acridine orange nucleic acid flow cytometry using the parameters of DNA, RNA, and proliferative activity. Correlation of the cytometric and morphologic data demonstrated that with increasing cytologic grade of lymphoma, the proliferative activity increased progressively and distinguished between grades (P less than 0.01). Immunologic studies identified B cell phenotype in 16 specimens, T cell in three, and true histiocytic lymphoma in one; one lymphoma had no cell markers (null cell). Six effusions proved to be inflammatory and reactive according to surface marker studies. Classification by cytologic characteristics showed good correlation with histologic classification performed previously. Immunologic study of cytologic specimens gave results identical to those achieved by frozen-section immunohistologic examination. Thus, immunologic and cytometric parameters can be readily performed on effusion specimens and aid in the diagnosis and classification of lymphomas.
非霍奇金淋巴瘤各亚型的生物学和临床异质性与形态学、免疫学及动力学特性的差异有关。关于淋巴瘤性积液中这些特征的综合研究尚未见报道。我们最近研究了26例临床疑似或确诊淋巴瘤患者的27份积液标本。对瑞氏-吉姆萨染色和巴氏染色的细胞学涂片、吖啶橙核酸流式细胞术以及使用一系列T和B细胞标志物抗体对细胞悬液进行的免疫过氧化物酶染色进行了评估，并与先前的组织学标本进行了比较。根据国际工作分类方案，通过细胞学特征对标本进行分类，并使用DNA、RNA和增殖活性参数通过吖啶橙核酸流式细胞术进行分类。细胞计数和形态学数据的相关性表明，随着淋巴瘤细胞学分级的增加，增殖活性逐渐增加，且在不同分级之间存在差异（P小于0.01）。免疫学研究在16份标本中鉴定出B细胞表型，3份中鉴定出T细胞表型，1份中鉴定出真性组织细胞淋巴瘤；1例淋巴瘤无细胞标志物（裸细胞）。根据表面标志物研究，6份积液被证明是炎性和反应性的。通过细胞学特征进行的分类与先前进行的组织学分类显示出良好的相关性。对细胞学标本的免疫学研究结果与冷冻切片免疫组织学检查的结果相同。因此，免疫学和细胞计数参数可很容易地在积液标本上进行，并有助于淋巴瘤的诊断和分类。
