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一种整合神经干细胞移植和功能性电刺激治疗大鼠下运动神经元疾病和损伤的策略。

A Therapeutic Strategy for Lower Motor Neuron Disease and Injury Integrating Neural Stem Cell Transplantation and Functional Electrical Stimulation in a Rat Model.

机构信息

Department of Human Enhancement and Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Department of Micro-Nano Mechanical Science and Engineering, Nagoya University Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

出版信息

Int J Mol Sci. 2022 Aug 6;23(15):8760. doi: 10.3390/ijms23158760.

DOI:10.3390/ijms23158760
PMID:35955890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9368824/
Abstract

Promising treatments for upper motor neuron disease are emerging in which motor function is restored by brain-computer interfaces and functional electrical stimulation. At present, such technologies and procedures are not applicable to lower motor neuron disease. We propose a novel therapeutic strategy for lower motor neuron disease and injury integrating neural stem cell transplantation with our new functional electrical stimulation control system. In a rat sciatic nerve transection model, we transplanted embryonic spinal neural stem cells into the distal stump of the peripheral nerve to reinnervate denervated muscle, and subsequently demonstrated that highly responsive limb movement similar to that of a healthy limb could be attained with a wirelessly powered two-channel neurostimulator that we developed. This unique technology, which can reinnervate and precisely move previously denervated muscles that were unresponsive to electrical stimulation, contributes to improving the condition of patients suffering from intractable diseases of paralysis and traumatic injury.

摘要

有前景的治疗方法正应用于上运动神经元疾病，这些治疗方法通过脑机接口和功能性电刺激来恢复运动功能。目前，这类技术和程序还不适用于下运动神经元疾病。我们提出了一种新的治疗策略，即将神经干细胞移植与我们新的功能性电刺激控制系统相结合，用于治疗下运动神经元疾病和损伤。在大鼠坐骨神经横断模型中，我们将胚胎脊髓神经干细胞移植到周围神经的远端残端，以重新支配去神经的肌肉，随后我们证明，使用我们开发的无线供电双通道神经刺激器，可以获得类似于健康肢体的高反应性肢体运动。这项独特的技术可以重新支配和精确移动以前对电刺激无反应的已去神经的肌肉，有助于改善瘫痪和创伤性损伤等难治性疾病患者的状况。

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