Beijing Institute of Radiation Medicine, Beijing 100850, China.
Int J Mol Sci. 2022 Aug 8;23(15):8810. doi: 10.3390/ijms23158810.
The mole fraction of deacetylated monomeric units in chitosan (CS) molecules is referred to as CS's degree of deacetylation (DD). In this study, 35 characteristic ions of CS were detected using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). The relative response intensity of 35 characteristic ion pairs using a single charge in nine CS samples with varying DDs was analyzed using 30 analytical methods. There was a good linear relationship between the relative response intensity of the characteristic ion pairs determined using ultrahigh performance (UP) LC-MS/MS and the DD of CS. The UPLC-MS/MS method for determining the DD of CS was unaffected by the sample concentration. The detection instrument has a wide range of application parameters with different voltages, high temperatures, and gas flow conditions. This study established a detection method for the DD of CS with high sensitivity, fast analysis, accuracy, stability, and durability.
壳聚糖（CS）分子中脱乙酰基单体单元的摩尔分数称为 CS 的脱乙酰度（DD）。本研究采用液相色谱-电喷雾电离-质谱联用（LC-ESI-MS/MS）技术检测到 35 种 CS 的特征离子。使用 30 种分析方法对 9 种具有不同 DD 的 CS 样品中 35 对特征单电荷离子对的相对响应强度进行了分析。采用超高效（UP）LC-MS/MS 法测定 CS 的特征离子对相对响应强度与 CS 的 DD 之间具有良好的线性关系。UPLC-MS/MS 法测定 CS 的 DD 不受样品浓度的影响。检测仪器具有较宽的应用参数范围，不同的电压、高温和气流条件。本研究建立了一种具有高灵敏度、快速分析、准确性、稳定性和耐用性的 CS 的 DD 检测方法。
