Beijing Institute of Radiation Medicine, Beijing 100850, China.
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Carbohydr Polym. 2024 Apr 1;329:121758. doi: 10.1016/j.carbpol.2023.121758. Epub 2024 Jan 2.
In this study, a novel and accurate quantitative analysis method for the direct determination of chitosan (CS) in aqueous solutions using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is presented. By detecting the mass spectrum response intensity of a series of CS characteristic ion pairs, the sample concentration (abscissa) was linearly fitted with the total ion current (TIC) response intensity of its characteristic ion pairs (ordinate). A reliable standard curve was derived for quantifying CS in the range of 125-4000 ng/mL. Under the detection conditions, this CS quantification method yielded acceptable specificity (no interference peak), linearity (with correlation coefficient (r) values >0.999), precision (acceptable limit RSD < 3 %, RSD < 6 %), accuracy (RE within the acceptable limits of ±5 %), and stability (acceptable limit RE within ±5 %, RSD < 3 %). Moreover, the applicability of measurement was verified when a series of substrates did not interact with CS in the solution. Results have verified the applicability of this method for determining CS content in different composites. This study provides a method for determining CS content with significant practical value and economic benefit.
本研究提出了一种新颖且准确的定量分析方法，可通过超高效液相色谱-串联质谱法（UPLC-MS/MS）直接测定水溶液中的壳聚糖（CS）。通过检测一系列 CS 特征离子对的质谱响应强度，将样品浓度（横坐标）与特征离子对的总离子流（TIC）响应强度（纵坐标）进行线性拟合。得出了一种可靠的标准曲线，用于在 125-4000ng/mL 范围内定量 CS。在检测条件下，该 CS 定量方法具有可接受的特异性（无干扰峰）、线性（相关系数（r）值>0.999）、精密度（可接受的 RSD<3%，RSD<6%）、准确度（RE 在可接受的±5%范围内）和稳定性（RE 在可接受的±5%范围内，RSD<3%）。此外，当一系列基质在溶液中不与 CS 相互作用时，验证了测量的适用性。结果验证了该方法在测定不同复合材料中 CS 含量的适用性。本研究提供了一种具有显著实用价值和经济效益的 CS 含量测定方法。
