Geng Lan, Lin Xinran, Liu Rang, Wu Jiahui, Luo Yongsheng, Sun Hongmei, Hou Zhenhui, Zhang Qiuju, Xu Chang, Li Xiao, Cao Canhui, Wang Tianren, Xia Xi
Center for Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518000, China.
Medical College, Shantou University Medical College, Shantou 515063, China.
J Clin Med. 2022 Jul 29;11(15):4417. doi: 10.3390/jcm11154417.
We explored the association between clinical outcomes and the cleavage rate of day-3 cleavage slow-growing embryos after overnight culture.
The data collected from 303 frozen embryo transfer (FET) cycles with 606 4-cell or 5-cell embryos cultured overnight (18-22 h) after thawing were analyzed. Based on the growth rate after the overnight culture, the embryos were divided into three groups: no embryo reaching eight cells (Group I), either one of the two embryos reaching eight cells (Group II), and both two embryos reaching eight cells or more (Group III). A statistical analysis of the different clinical outcomes from the three groups was performed.
Biochemical pregnancy rate (OR 3.22; = 0.001), implantation rate (OR 2.44; = 0.002), clinical pregnancy rate (OR 3.04; = 0.001), ongoing pregnancy rate (OR 3.14; = 0.001), and live birth rate (OR 2.78; = 0.004) were significantly higher in Group III as compared to Group I. Group II had a significantly higher biochemical pregnancy rate (OR 2.02; = 0.013) and implantation rate (OR 1.77; = 0.019) than Group I.
The capability of day-3 cleavage slow-growing embryos to reach eight cells, especially that of two embryos reaching eight cells by overnight culture, appear to result in a better pregnancy outcome.
我们探讨了过夜培养后第3天卵裂缓慢生长胚胎的卵裂率与临床结局之间的关联。
分析了从303个冻融胚胎移植（FET）周期收集的数据，这些周期中有606个4细胞或5细胞胚胎在解冻后过夜培养（18 - 22小时）。根据过夜培养后的生长率，将胚胎分为三组：没有胚胎发育到8细胞（第一组），两个胚胎中有一个发育到8细胞（第二组），以及两个胚胎都发育到8细胞或更多（第三组）。对三组不同的临床结局进行了统计分析。
与第一组相比，第三组的生化妊娠率（OR 3.22；P = 0.001）、着床率（OR 2.44；P = 0.002）、临床妊娠率（OR 3.04；P = 0.001）、持续妊娠率（OR 3.14；P = 0.001）和活产率（OR 2.78；P = 0.004）显著更高。第二组的生化妊娠率（OR 2.02；P = 0.013）和着床率（OR 1.77；P = 0.019）显著高于第一组。
第3天卵裂缓慢生长胚胎发育到8细胞的能力，尤其是两个胚胎通过过夜培养都发育到8细胞，似乎会带来更好的妊娠结局。
