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宾夕法尼亚双歧杆菌亚种的细胞表面疏水性。

Cell surface hydrophobicity of Bifidobacterium bifidum subsp. pennsylvanicum.

作者信息

Op den Camp H J, Oosterhof A, Veerkamp J H

出版信息

Antonie Van Leeuwenhoek. 1985;51(3):303-12. doi: 10.1007/BF02439939.

DOI:10.1007/BF02439939
PMID:4091536
Abstract

The possible role of lipoteichoic acid with respect to cell surface properties of Bifidobacterium bifidum subsp. pennsylvanicum was studied. Standard suspensions of bacteria were mixed with octane or xylene. B. bifidum subsp. pennsylvanicum was shown to possess a strongly hydrophobic cell surface. Hydrophobicity of the bacteria could be reduced by treatment with trypsin, pepsin (at pH 4.5), HCl and penicillin. The latter treatment resulted in an increased excretion of lipoteichoic acid. Albumin was capable of inhibiting the adherence to octane when it was present in the assay buffer. The data suggest that both protein and lipoteichoic acid may be involved in cell surface hydrophobicity. A great divergence in cell surface properties was observed within the genus Bifidobacterium.

摘要

研究了磷壁酸对宾夕法尼亚双歧杆菌亚种细胞表面特性的可能作用。将细菌的标准悬浮液与辛烷或二甲苯混合。结果表明,宾夕法尼亚双歧杆菌亚种具有高度疏水的细胞表面。用胰蛋白酶、胃蛋白酶(pH 4.5)、盐酸和青霉素处理可降低细菌的疏水性。后一种处理导致磷壁酸排泄增加。当测定缓冲液中存在白蛋白时,它能够抑制对辛烷的黏附。数据表明,蛋白质和磷壁酸可能都与细胞表面疏水性有关。在双歧杆菌属内观察到细胞表面特性存在很大差异。

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本文引用的文献

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The isolation and cultivation of Lactobacillus bifidus; a comparison of branched and unbranched strains.双歧杆菌的分离与培养;分支菌株与非分支菌株的比较
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