College of Food Science and Technology, Henan Agricultural University, 63#Agricultural Road, Zhengzhou 450000, China.
Department for Food Engineering and Hydromechanics, School of Engineering and Technology, Mongolian State University of Life Sciences, Zaisan-53, Ulaanbaatar 17024, Mongolia.
Nutrients. 2022 Jul 28;14(15):3102. doi: 10.3390/nu14153102.
Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.
许多研究人员发现，Pb 暴露会导致机体组织氧化应激损伤。黑大豆肽（BSP）具有多种生理功能，尤其在氧化应激方面。然而，BSP 对 Pb 诱导的 PC12 细胞氧化应激损伤的缓解作用尚未明确。本研究采用 CCK8 检测细胞活力。采用生化试剂盒检测 ROS、GSH/GSSG、MDA、SOD、CAT、GPx 和 GR 等氧化应激指标。Western blot 法检测 Keap1、Nrf2 和 TXNIP 蛋白表达。与对照组相比，Pb 降低了 PC12 细胞的活力。然而，BSP 处理可显著提高 Pb 暴露诱导的 PC12 细胞活力（p＜0.05）。Pb 可增加 MDA 和 ROS 的含量，但降低 CAT、SOD、GR、GPx 和 GSH/GSSG 的含量，而 BSP 可减轻这种情况（p＜0.05）。Pb 可增强 Keap1 和 TXNIP 蛋白的表达，但降低 Nrf2 表达。相比之下，BSPs 逆转了这种现象（p＜0.05）。BSPs 可通过 Keap1/Nrf2/TXNIP 信号通路缓解 Pb 诱导的 PC12 细胞氧化应激损伤。
