Li Ning, Wen Liuding, Wang Fangyu, Li Tiange, Zheng Haodan, Wang Tianlin, Qiao Mingwu, Huang Xianqing, Song Lianjun, Bukyei Erkigul, Li Mingming
College of Food Science and Technology, Henan Agricultural University, Zhengzhou, China.
Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Front Nutr. 2022 Aug 11;9:964938. doi: 10.3389/fnut.2022.964938. eCollection 2022.
Lead poisoning causes an oxidative stress response - a key "bridge" connecting various pathways - in the human body. Oxidative stress usually implies an imbalance between pro-oxidants and antioxidants. Moreover, Nrf2, Keap1, and TXNIP proteins play an essential role in oxidative stress. Some studies showed that pea peptides could alleviate the oxidative stress response. However, the effect and mechanism of pea peptide on oxidative stress response induced by lead in PC12 cells has not been reported.
Investigating the effect and mechanism of pea peptides in alleviating oxidative damage in PC12 cells induced by lead.
In this study, cell viability was measured by CCK8 (Cell Counting Kit-8). Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), reactive oxygen species (ROS), and lipid peroxidation (MDA) were measured using the corresponding Biochemical kits. The Keap1, Nrf2, and TXNIP protein expressions were tested using Western blot.
Pea peptides PP3, PP4, and PP6 could reverse the decrease of cell viability caused by lead exposure ( < 0.05), the elevation of ROS and MDA caused by lead exposure, and the decrease of CAT, SOD, GR, GPx, and GSH/GSSG caused by lead exposure ( < 0.05). Moreover, PP3, PP4, and PP6 could reduce the elevated expression of Keap1 and TXNIP caused by lead exposure; and increase the expression of Nrf2 ( < 0.05).
PP3, PP4, and PP6 can alleviate lead-induced oxidative stress damage in PC12 cells, and the Nrf2/Keap1/TXNIP signaling pathway may play an essential role in this process.
铅中毒会在人体引发氧化应激反应，这是连接各种途径的关键“桥梁”。氧化应激通常意味着促氧化剂和抗氧化剂之间的失衡。此外，Nrf2、Keap1和TXNIP蛋白在氧化应激中起重要作用。一些研究表明豌豆肽可以减轻氧化应激反应。然而，豌豆肽对铅诱导的PC12细胞氧化应激反应的影响及机制尚未见报道。
研究豌豆肽减轻铅诱导的PC12细胞氧化损伤的作用及机制。
本研究中，采用CCK8（细胞计数试剂盒-8）检测细胞活力。使用相应的生化试剂盒检测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPx）、活性氧（ROS）和脂质过氧化（MDA）。采用蛋白质免疫印迹法检测Keap1、Nrf2和TXNIP蛋白表达。
豌豆肽PP3、PP4和PP6可逆转铅暴露引起的细胞活力下降（P<0.05）、铅暴露引起的ROS和MDA升高，以及铅暴露引起的CAT、SOD、GR、GPx和GSH/GSSG下降（P<0.05）。此外，PP3、PP4和PP6可降低铅暴露引起的Keap1和TXNIP表达升高；并增加Nrf2的表达（P<0.05）。
PP3、PP4和PP6可减轻铅诱导的PC12细胞氧化应激损伤，Nrf2/Keap1/TXNIP信号通路可能在此过程中起重要作用。
