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特定植物酚类物质在细胞实验中的直接和间接抗氧化作用

Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays.

作者信息

Treml Jakub, Večeřová Petra, Herczogová Petra, Šmejkal Karel

机构信息

Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackeho tr. 1946/1, 612 00 Brno, Czech Republic.

Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackeho tr. 1946/1, 612 00 Brno, Czech Republic.

出版信息

Molecules. 2021 Apr 26;26(9):2534. doi: 10.3390/molecules26092534.

DOI:10.3390/molecules26092534
PMID:33926137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8123583/
Abstract

: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. : The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. : Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. : Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.

摘要

氧化应激是许多疾病病理生理学中的关键因素。本研究旨在通过细胞实验验证所选植物酚类物质的抗氧化活性，并确定其直接或间接作用。采用细胞抗氧化分析（CAA）进行直接清除实验。在间接方法中，观察了每种受试物质对所选抗氧化酶的基因和蛋白表达及活性的影响。还进行了一项关于Nrf2-ARE途径激活的实验。使用葡萄糖氧化应激保护实验来测定每种化合物的总体效果。在受试化合物中，毛蕊花糖苷表现出最高的直接清除活性，且对抗氧化酶的表达没有影响。它仅增加了过氧化氢酶的活性。二氢桑色素在直接抗氧化实验中的活性较低，但对酶表达和过氧化氢酶活性有积极影响。桑色素在CAA实验中未表现出抗氧化活性。同样， pomiferin只有轻微的抗氧化活性，且具有相当的细胞毒性。在所选的四种酚类物质中，只有毛蕊花糖苷和二氢桑色素在细胞实验中表现出抗氧化作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/4b7e6e7e279f/molecules-26-02534-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/f4e9f3aefded/molecules-26-02534-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/6d0f182a976a/molecules-26-02534-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/4f6841539758/molecules-26-02534-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/73751256a639/molecules-26-02534-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/0f37a7a1c2af/molecules-26-02534-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/dcadf4247d36/molecules-26-02534-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/7ad40480ad8c/molecules-26-02534-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/7f13bdfa4d39/molecules-26-02534-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/4b7e6e7e279f/molecules-26-02534-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/f4e9f3aefded/molecules-26-02534-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/6d0f182a976a/molecules-26-02534-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/4f6841539758/molecules-26-02534-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/73751256a639/molecules-26-02534-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/0f37a7a1c2af/molecules-26-02534-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/dcadf4247d36/molecules-26-02534-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/7ad40480ad8c/molecules-26-02534-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/7f13bdfa4d39/molecules-26-02534-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7be/8123583/4b7e6e7e279f/molecules-26-02534-g009.jpg

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