Raghunandan K, Tanwar Jatin, Patil Shivanagouda N, Chandra Ajay Kumar, Tyagi Sandhya, Agarwal Priyanka, Mallick Niharika, Murukan Niranjana, Kumari Jyoti, Sahu Tanmaya Kumar, Jacob Sherry R, Kumar Atul, Yadav Suresh, Nyamgoud Sneha, Singh Amit Kumar, Jha Shailendra Kumar
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India.
Plants (Basel). 2022 Jul 28;11(15):1965. doi: 10.3390/plants11151965.
Wheat leaf rust caused by Eriks is an important disease that causes yield losses of up to 40% in susceptible varieties. Tetraploid emmer wheat ( ssp. ) commonly called Khapli wheat in India, is known to have evolved from wild emmer ( var. ), and harbors a good number of leaf rust resistance genes. In the present study, we are reporting on the screening of one hundred and twenty-three dicoccum wheat germplasm accessions against the leaf rust pathotype 77-5. Among these, an average of 45.50% of the germplasms were resistant, 46.74% were susceptible, and 8.53% had mesothetic reactions. Further, selected germplasm lines with accession numbers IC138898, IC47022, IC535116, IC535133, IC535139, IC551396, and IC534144 showed high level of resistance against the eighteen prevalent pathotypes. The infection type varied from ";", ";N", ";N1" to ";NC". PCR-based analysis of the resistant dicoccum lines with SSR marker linked to the gene, a leaf rust resistance gene effective against all the prevalent pathotypes of leaf rust in India and identified from a var. germplasm, indicated that is not present in the selected accessions. Moreover, we have also generated 35K SNP genotyping data of seven lines and the susceptible control, Mandsaur Local, to study their relationships. The GDIRT tool based on homozygous genotypic differences revealed that the seven genotypes are unique to each other and may carry different resistance genes for leaf rust.
由埃里克氏菌引起的小麦叶锈病是一种重要病害，在易感品种中可导致高达40%的产量损失。四倍体二粒小麦（亚种）在印度通常被称为卡普利小麦，已知其由野生二粒小麦（变种）进化而来，并且含有大量叶锈病抗性基因。在本研究中，我们报告了针对叶锈病菌株77-5对123份二粒小麦种质资源的筛选情况。其中，平均45.50%的种质资源具有抗性，46.74%易感，8.53%表现为中间型反应。此外，选择的种质系，其种质编号为IC138898、IC47022、IC535116、IC535133、IC535139、IC551396和IC534144，对18种流行菌株表现出高抗性水平。感染类型从“;”、“;N”、“;N1”到“;NC”不等。利用与Lr34基因连锁的SSR标记对抗性二粒小麦品系进行基于PCR的分析，Lr34是一个对印度所有流行叶锈病菌株均有效的叶锈病抗性基因，并且是从一个野生二粒小麦变种种质中鉴定出来的，结果表明所选种质中不存在Lr34基因。此外，我们还生成了7个品系和易感对照品种曼德索尔本地种的35K SNP基因分型数据，以研究它们之间的关系。基于纯合基因型差异的GDIRT工具显示，这7个基因型彼此独特，可能携带不同的叶锈病抗性基因。
