Carvalho Thais Louise G, Rosman Aline C, Grativol Clícia, de M Nogueira Eduardo, Baldani José Ivo, Hemerly Adriana S
Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, RJ, Brazil.
Laboratório de Química e Funções de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes 28015-622, RJ, Brazil.
Plants (Basel). 2022 Jul 29;11(15):1971. doi: 10.3390/plants11151971.
Sugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more efficient BNF genotypes.
甘蔗是一种具有重要经济价值的作物，可用于生产燃料乙醇。已从甘蔗组织中分离出固氮细菌，且这些细菌不会引起明显的植物解剖结构变化或病害症状。这些细菌可通过促进根系生长和提高作物产量对植物有益。在不同基因型中观察到了不同的生物固氮（BNF）速率。这项工作的目的是对两种具有对比性BNF效率的模式基因型进行全面的分子和生理分析，以揭示在与固氮细菌自然共生过程中差异调控的植物基因。对基因型SP70 - 1143（高BNF）和Chunee（低BNF）的RNA样本进行了新一代测序。差异转录组分析表明，在两种具有对比性BNF的基因型中，包括氮代谢、激素调节和细菌识别在内的几个途径受到了差异调控。生理分析，如固氮酶和GS活性定量、细菌定殖、生长素反应和根系结构评估，支持了转录组表达分析。基因型之间观察到的差异可能至少部分解释了BNF贡献的差异。一些已鉴定的基因可能参与了有益共生的关键调控过程，并可进一步用作获得更高效BNF基因型的工具。
