Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, China.
Molecules. 2022 Jul 27;27(15):4796. doi: 10.3390/molecules27154796.
Pomegranate peel extract (PPE), which is abundant in polyphenols, holds immerse prospects for the treatment of airway infection. In this study, water and ethanol of 30%, 50%, and 80% were used to prepare PPE. A total of 18 phenols belonging to 8 categories of polyphenols were identified in PPE by HPLC-MS/MS. The PPE from the four extraction solvents possessed different antioxidant, antibacterial, and anti-inflammatory activities. Principal component analysis revealed that though total flavonoids (TFs), total polyphenols (TPs), and total tannins (TTs) were responsible for the reducing power of PPE, only TFs contributed to the effect of PPE in inhibiting lipid membrane peroxidation. TPs, TTs, and punicalagin were positively correlated with the antibacterial strength against while TTs alone contributed to the inhibition of methicillin-resistant , implying the crucial role of TT in suppressing bacteria. Meanwhile, TTs was associated with the prevention of IL-6 release. The PPE with higher contents of TPs, TTs, and punicalagin had a weaker capacity to decrease nitric oxide secretion. PPE of 30% ethanol gained the highest integrated score due to its stronger antioxidant, antibacterial, and anti-inflammatory activities. It is a suitable candidate for the therapy of respiratory tract infection.
石榴皮提取物（PPE）富含多酚，在治疗气道感染方面具有广阔的前景。在本研究中，采用水和 30%、50%和 80%的乙醇来制备 PPE。通过 HPLC-MS/MS 在 PPE 中鉴定出 18 种属于 8 类多酚的酚类物质。四种提取溶剂的 PPE 具有不同的抗氧化、抗菌和抗炎活性。主成分分析表明，虽然总黄酮（TFs）、总多酚（TPs）和总单宁（TTs）是 PPE 还原能力的原因，但只有 TFs 对 PPE 抑制脂质膜过氧化的作用有贡献。TPs、TTs 和安石榴甙与对 的抗菌强度呈正相关，而 TTs 单独有助于抑制耐甲氧西林金黄色葡萄球菌，表明 TT 在抑制细菌方面起着关键作用。同时，TTs 与预防白细胞介素-6 的释放有关。TPs、TTs 和安石榴甙含量较高的 PPE 降低一氧化氮分泌的能力较弱。由于具有更强的抗氧化、抗菌和抗炎活性，30%乙醇的 PPE 获得了最高的综合评分。它是治疗呼吸道感染的合适候选药物。
