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基质辅助激光解吸/电离飞行时间质谱（MALDI TOF MS）与VITEK 2在常规微生物鉴定中的评估

Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification.

作者信息

Madhavan Anitha, Sachu Arun, Sethuraman Nandini, Kumar Anil, Vasudevapanicker Jayalakshmi

机构信息

Department of Microbiology, Government TD Medical College, Alappuzha, Kerala, India.

Department of Microbiology, Believers Church Medical College, Thiruvalla, Kerala, India.

出版信息

Ghana Med J. 2021 Dec;55(4):308-310. doi: 10.4314/gmj.v55i4.12.

DOI:10.4314/gmj.v55i4.12
PMID:35957934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9334958/
Abstract

BACGROUND

Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.

OBJECTIVES

To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.

METHODS

The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.

RESULTS

MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.

CONCLUSION

MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.

FUNDING

State Board of Medical Research (SBMR).

摘要

背景

微生物鉴定采用表型方法。VITEK-2和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）目前在实验室中使用得越来越多。

目的

比较和评估MALDI-TOF MS和VITEK-2在常规微生物鉴定中的实用性。

方法

比较MALDI-TOF MS和VITEK 2在鉴定微生物方面的性能。

结果

MALDI-TOF MS和VITEK-2在属水平上分别正确鉴定了96%（96/100）和97%（97/100）的分离株。

结论

MALDI TOF MS和VITEK -2的鉴定率和错误率相当。MALDI TOF能快速缩短周转时间，这在临床微生物学领域是一个重大的变革。

资助

国家医学研究委员会（SBMR）。

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