Dey Supantha, Shahrear Sazzad, Afroj Zinnia Maliha, Tajwar Ahnaf, Islam Abul Bashar Mir Md Khademul
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Department of Pharmacy, East West University, Dhaka, Bangladesh.
Bioinform Biol Insights. 2022 Aug 6;16:11779322221115535. doi: 10.1177/11779322221115535. eCollection 2022.
B13 strain is a rod-shaped gram-negative bacterium that belongs to the family. It can cause respiratory and urinary tract infections, and is responsible for several outbreaks in hospitals. has become an important pathogen and an emerging global threat because of its opportunistic and multidrug resistant ability. However, little knowledge is present about a large portion of its proteins and functions. Therefore, functional annotation of the hypothetical proteins (HPs) can provide an improved understanding of this organism and its virulence activity. The workflow in the study included several bioinformatic tools which were utilized to characterize functions, family and domains, subcellular localization, physiochemical properties, and protein-protein interactions. The B13 strain has overall 604 HPs, among which 78 were functionally annotated with high confidence. Several proteins were identified as enzymes, regulatory, binding, and transmembrane proteins with essential functions. Furthermore, 23 HPs were predicted to be virulent factors. These virulent proteins are linked to pathogenesis with their contribution to biofilm formation, quorum sensing, 2-component signal transduction or secretion. Better knowledge about the HPs' characteristics and functions will provide a greater overview of the proteome. Moreover, it will help against in neonatal intensive care unit (NICU) outbreaks and nosocomial infections.
B13菌株是一种杆状革兰氏阴性细菌，属于该菌属。它可引起呼吸道和泌尿道感染，并在医院引发多起疫情。由于其具有机会致病性和多重耐药性，已成为一种重要的病原体和新出现的全球威胁。然而，对于其大部分蛋白质及其功能了解甚少。因此，对假定蛋白质（HPs）进行功能注释有助于更好地了解这种生物体及其毒力活性。该研究中的工作流程包括多种生物信息学工具，用于表征功能、家族和结构域、亚细胞定位、理化性质以及蛋白质-蛋白质相互作用。B13菌株共有604个假定蛋白质，其中78个已被高可信度地进行了功能注释。一些蛋白质被鉴定为具有重要功能的酶、调节蛋白、结合蛋白和跨膜蛋白。此外，预测有23个假定蛋白质是毒力因子。这些毒力蛋白通过其对生物膜形成、群体感应、双组分信号转导或分泌的作用与发病机制相关。对假定蛋白质的特征和功能有更深入的了解将有助于更全面地了解蛋白质组。此外，这将有助于应对新生儿重症监护病房（NICU）的疫情爆发和医院感染。
