Yu Kuaiyun, Zhu Heng
Department of General Surgery, Yantaishan Hospital Yantai 264001, Shandong, China.
Department of Digestive, The Fourth People's Hospital of Ji'nan Ji'nan 250031, Shandong, China.
Am J Transl Res. 2022 Jul 15;14(7):5050-5058. eCollection 2022.
MiR-762 has been confirmed as a tumor promoter in multiple tumors, while few reports illustrate its role in gastric cancer (GC). Thus, this research aimed to investigate whether miR-762 is involved in GC development.
MiR-762 expression in the tumor tissues from GC patients and GC cell lines was analyzed by qRT-PCR. The assays including CCK-8, transwell, and flow cytometry were performed to reveal the functions of miR-762 in GC. The target genes of miR-762 were searched by online databases, and then were verified by dual-luciferase reporter assay. Western blot was performed to investigate the activation of PI3K/AKT and Hippo pathways in GC.
MiR-762 was aberrantly upregulated in the tumor tissues and cell lines, and miR-762 silencing could effectively reduce the viability and promote apoptosis of GC cell lines. The study identified LZTS1 as a target gene of miR-762. It was also found that the effects of miR-762 on GC cells could be reversed by LZTS1, and miR-762 could upregulate the activation of the PI3K/AKT pathway but inhibit the Hippo pathway by targeting LZTS1.
MiR-762 activates PI3K/AKT and suppresses Hippo pathways to boost GC proliferation and invasion by targeting LZTS1.
MiR - 762已被证实为多种肿瘤中的肿瘤促进因子，而关于其在胃癌（GC）中作用的报道较少。因此，本研究旨在探讨MiR - 762是否参与GC的发生发展。
采用qRT - PCR分析GC患者肿瘤组织和GC细胞系中MiR - 762的表达。进行CCK - 8、transwell和流式细胞术等实验，以揭示MiR - 762在GC中的功能。通过在线数据库搜索MiR - 762的靶基因，然后用双荧光素酶报告基因检测进行验证。采用蛋白质免疫印迹法研究GC中PI3K/AKT和Hippo信号通路的激活情况。
MiR - 762在肿瘤组织和细胞系中异常上调，沉默MiR - 762可有效降低GC细胞系的活力并促进其凋亡。该研究确定LZTS1为MiR - 762的靶基因。还发现LZTS1可逆转MiR - 762对GC细胞的影响，且MiR - 762可通过靶向LZTS1上调PI3K/AKT信号通路的激活，但抑制Hippo信号通路。
MiR - 762通过靶向LZTS1激活PI3K/AKT信号通路并抑制Hippo信号通路，从而促进GC的增殖和侵袭。
