Lu Ling, Ma Yan, Deng Jie, Xie Jiaqiong, Huang Chaolin
Department of Gynaecology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People's Republic of China.
Department of Obstetrics, The First Affiliated Hospital of Chengdu Medical College, Chengdu, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Aug 4;15:2335-2343. doi: 10.2147/DMSO.S377041. eCollection 2022.
This study aimed to investigate ATG7 levels in pregnant women with and without gestational diabetes mellitus (GDM) and explore the potential associations between ATG7 levels and GDM.
This was a cross-sectional study conducted in a large tertiary hospital in Chengdu, China. The ATG7 levels in pregnant women at between 24 and 28 weeks of gestation with (n=84) and without GDM (n=649) were measured by using an ELISA kit. Glucose, HbA1c, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by an automatic biochemistry analyser. The homeostasis model assessment of insulin resistance (HOMA-IR) and insulin secretion (HOMA-β) were calculated according to published formulas. The associations of ATG7 levels with laboratory parameters, GDM, and insulin resistance were evaluated using correlation analysis or a regression model.
The ATG7 levels were significantly lower in pregnant women with GDM than in those without GDM. The correlation analyses found that ATG7 levels correlated positively with HOMA-β but correlated negatively with HOMA-IR, oral glucose tolerance test (OGTT) glucose levels, TGs, and LDL-C. There were no significant correlations between ATG7 levels and HbA1c, HDL-C, or TC. After adjusting for potential confounders, lower ATG7 levels were shown to be associated with a higher risk of GDM. Furthermore, multiple linear regression analyses showed that ATG7 levels were negatively associated with insulin resistance.
ATG7 levels are significantly lower in pregnant women with GDM than in those without GDM, and lower ATG7 levels are associated with a higher risk of GDM. ATG7 levels were negatively associated with insulin resistance. Autophagy deficiency, which is caused by lower ATG7 levels, may be the underlying mechanism that mediates insulin resistance in the development of GDM.
本研究旨在调查患有和未患有妊娠期糖尿病（GDM）的孕妇体内自噬相关基因7（ATG7）水平，并探讨ATG7水平与GDM之间的潜在关联。
这是一项在中国成都一家大型三级医院开展的横断面研究。使用酶联免疫吸附测定（ELISA）试剂盒测量妊娠24至28周患有GDM（n = 84）和未患有GDM（n = 649）的孕妇的ATG7水平。通过自动生化分析仪测量血糖、糖化血红蛋白（HbA1c）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平。根据已发表的公式计算胰岛素抵抗的稳态模型评估（HOMA-IR）和胰岛素分泌（HOMA-β）。使用相关分析或回归模型评估ATG7水平与实验室参数、GDM和胰岛素抵抗之间的关联。
患有GDM的孕妇的ATG7水平显著低于未患有GDM的孕妇。相关分析发现，ATG7水平与HOMA-β呈正相关，但与HOMA-IR、口服葡萄糖耐量试验（OGTT）血糖水平、TG和LDL-C呈负相关。ATG7水平与HbA1c、HDL-C或TC之间无显著相关性。在调整潜在混杂因素后，较低的ATG7水平与GDM风险较高相关。此外，多元线性回归分析表明，ATG7水平与胰岛素抵抗呈负相关。
患有GDM的孕妇的ATG7水平显著低于未患有GDM的孕妇，且较低的ATG7水平与GDM风险较高相关。ATG7水平与胰岛素抵抗呈负相关。由较低的ATG7水平引起的自噬缺陷可能是GDM发生发展中介导胰岛素抵抗的潜在机制。
