Iravani Kamyar, Mehrabani Davood, Doostkam Aida, Azarpira Negar, Iranpour Pooya, Bahador Mohsen, Mehravar Soheila
Otolaryngology Research Center, Department of Otolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran.
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr Ther Res Clin Exp. 2022 Jul 22;97:100682. doi: 10.1016/j.curtheres.2022.100682. eCollection 2022.
Stenosis and scar formation after repair of laryngeal tissue defects are serious problems that can significantly influence a patient's quality of life.
In this study, we evaluated the use of magnetic resonance imaging to assess the efficacy of adipose tissue-derived mesenchymal stem cells (ASCs) on cartilaginous regeneration in an experimental rabbit model.
Ten male white Dutch rabbits each had a 5 mm cartilaginous defect created surgically in the right and left thyroid lamina. On the right side, ASCs labeled with iron oxide particles were infused. As a control, the left side was left untreated. Repair of the defects were then evaluated by direct observation, histological evaluation, and magnetic resonance imaging monitoring done on days 1, 7, 14, and 28.
Histological examination revealed that compared with control, transplanted ASCs significantly increased cartilage regeneration ( ˂ 0.001), reduced inflammation ( ˂ 0.001), and fibrosis ( = 0.050). Magnetic resonance imaging tracking showed accurate placement and viability of the infused ASCs, as evidenced by low signal intensity onT2 weighted images at the level of the right thyroid cartilage.
Infusion of ASCs improved laryngeal regeneration of surgically induced cartilaginous defects while decreasing fibrous tissue formation in this in vivo rabbit model. Furthermore, magnetic resonance imaging was shown to be a useful, noninvasive method to track correct ASCs placement and viability in cartilage regeneration in this animal model.
喉组织缺损修复后的狭窄和瘢痕形成是严重问题，会显著影响患者生活质量。
在本研究中，我们评估了磁共振成像在实验性兔模型中评估脂肪组织来源的间充质干细胞（ASC）对软骨再生疗效的应用。
10只雄性荷兰白兔，在左右甲状软骨板上通过手术制造5毫米的软骨缺损。右侧注入用氧化铁颗粒标记的ASC。作为对照，左侧不做处理。然后在第1、7、14和28天通过直接观察、组织学评估和磁共振成像监测来评估缺损的修复情况。
组织学检查显示，与对照组相比，移植的ASC显著增加了软骨再生（˂0.001），减少了炎症（˂0.001）和纤维化（ = 0.050）。磁共振成像追踪显示注入的ASC定位准确且具有活性，右侧甲状软骨水平的T2加权图像上低信号强度证明了这一点。
在这个体内兔模型中，注入ASC改善了手术诱导的软骨缺损的喉再生，同时减少了纤维组织形成。此外，磁共振成像被证明是一种有用的非侵入性方法，可追踪ASC在该动物模型软骨再生中的正确定位和活性。
