Chu Wenhui, Wang Pan, Ma Zhe, Peng Lin, Wang Zongmin, Chen Zilin
School of Life Science Taizhou University Taizhou China.
Traditional Chinese Medicine Industry Development and Promotion Center of Pan'an County Pan'an China.
Food Sci Nutr. 2022 Apr 1;10(8):2620-2630. doi: 10.1002/fsn3.2867. eCollection 2022 Aug.
Utilization of the biological macromolecule polysaccharide (DOP) as a functional ingredient is limited by its high intrinsic viscosity and molecular weight. The goal of the present study was to improve rheological properties of DOP by ultrasonic treatment. Such a treatment resulted in the degradation of DOP and consequent reduction of rheological properties. Among DOP samples treated with ultrasonication at low (L), medium (M), and high (H) power intensities (25, 50, 75 w/cm), M-DOP displayed the highest reactive oxygen species (ROS) and reactive nitrogen species (RNS) radical scavenging activity in vitro. In a mouse D-galactose (D-Gal)-induced aging model, M-DOP significantly increased activities of antioxidant enzymes and reduced levels of pro-inflammatory cytokines in liver. Real-time polymerase chain reaction (RT-PCR) analysis indicated that M-DOP upregulated messenger RNA (mRNA) expression of anti-inflammatory/antioxidant proteins such as Nrf2 (nuclear factor erythroid 2-related factor), hemeoxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase (NQO1) in liver. In summary, M-DOP displayed a strong radical scavenging activity in vitro, and ameliorated liver injury in the mouse aging model through the promotion of Nrf2/HO-1/NQO1 signaling pathway.
生物大分子多糖（DOP）作为一种功能成分的应用受到其高特性粘度和分子量的限制。本研究的目的是通过超声处理改善DOP的流变学性质。这种处理导致DOP降解，进而降低其流变学性质。在低（L）、中（M）、高（H）功率强度（25、50、75 w/cm）超声处理的DOP样品中，M-DOP在体外表现出最高的活性氧（ROS）和活性氮（RNS）自由基清除活性。在小鼠D-半乳糖（D-Gal）诱导的衰老模型中，M-DOP显著提高了肝脏中抗氧化酶的活性，并降低了促炎细胞因子的水平。实时聚合酶链反应（RT-PCR）分析表明，M-DOP上调了肝脏中抗炎/抗氧化蛋白如Nrf2（核因子红细胞2相关因子）、血红素加氧酶-1（HO-1）和NAD（P）H：醌氧化还原酶（NQO1）的信使核糖核酸（mRNA）表达。总之，M-DOP在体外表现出较强的自由基清除活性，并通过促进Nrf2/HO-1/NQO1信号通路改善了小鼠衰老模型中的肝损伤。
