Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China.
Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka 410001, Nigeria.
Nutrients. 2023 Jun 6;15(12):2641. doi: 10.3390/nu15122641.
is one of the most widely used medicinal herbs, especially in Asia. In recent times, the polysaccharide content of has garnered attention due to the numerous reports of its medicinal properties, such as anticancer, antioxidant, anti-diabetic, hepatoprotective, neuroprotective, and anti-aging activities. However, few reports of its anti-aging potential are available. Due to high demand, the wild is scarce; hence, alternative cultivation methods are being employed. In this study, we used the model to investigate the anti-aging potential of polysaccharides extracted from (DOP) grown in three different environments; tree (TR), greenhouse (GH), and rock (RK). Our findings showed that at 1000 µg/mL, GH-DOP optimally extended the mean lifespan by 14% and the maximum lifespan by 25% ( < 0.0001). TR-DOP and RK-DOP did not extend their lifespan at any of the concentrations tested. We further showed that 2000 µg/mL TR-DOP, GH-DOP, or RK-DOP all enhanced resistance to HO-induced stress ( > 0.05, < 0.01, and < 0.01, respectively). In contrast, only RK-DOP exhibited resistance ( < 0.01) to thermal stress. Overall, DOP from the three sources all increased HSP-4::GFP levels, indicating a boost in the ability of the worms to respond to ER-related stress. Similarly, DOP from all three sources decreased α-synuclein aggregation; however, only GH-DOP delayed β-amyloid-induced paralysis ( < 0.0001). Our findings provide useful information on the health benefits of DOP and also provide clues on the best practices for cultivating for maximum medicinal applications.
是最广泛使用的药用植物之一，特别是在亚洲。近年来，由于其具有多种药用特性的报道，如抗癌、抗氧化、抗糖尿病、保肝、神经保护和抗衰老作用， 的多糖含量引起了人们的关注。然而，关于其抗衰老潜力的报道很少。由于需求量大，野生 稀缺；因此，正在采用替代种植方法。在这项研究中，我们使用 模型研究了在三种不同环境下生长的 （DOP）中提取的多糖的抗衰老潜力；树（TR）、温室（GH）和岩石（RK）。我们的研究结果表明，在 1000 µg/mL 时，GH-DOP 最佳地将平均寿命延长了 14%，最大寿命延长了 25%（<0.0001）。在任何测试浓度下，TR-DOP 和 RK-DOP 都没有延长其寿命。我们进一步表明，2000 µg/mL 的 TR-DOP、GH-DOP 或 RK-DOP 都增强了对 HO 诱导应激的抗性（分别为>0.05、<0.01 和<0.01）。相比之下，只有 RK-DOP 对热应激表现出抗性（<0.01）。总体而言，来自这三种来源的 DOP 都增加了 HSP-4::GFP 水平，表明蠕虫应对 ER 相关应激的能力得到了提高。同样，来自这三种来源的 DOP 都减少了 α-突触核蛋白聚集；然而，只有 GH-DOP 延迟了 β-淀粉样蛋白诱导的瘫痪（<0.0001）。我们的研究结果提供了关于 DOP 健康益处的有用信息，并且还为最佳的 种植实践提供了线索，以实现最大的药用应用。
