Cherry W B, Pittman B, Harris P P, Hebert G A, Thomason B M, Thacker L, Weaver R E
J Clin Microbiol. 1978 Sep;8(3):329-38. doi: 10.1128/jcm.8.3.329-338.1978.
Antisera and fluorescein isothiocyanate conjugates prepared for five strains of the Legionnaires bacteria were tested in both homologous and heterologous staining reactions with 10 isolates of the organism from patients in seven geographic areas. The strains were related but not identical as judged by the results of direct immunofluorescence staining. The conjugates were successfully used to detect Legionnaires disease bacteria in Formalin-fixed lung scrapings, in histological sections, and in fresh lung tissue obtained at biopsy or autopsy. In addition, the labeled antibodies are valuable for staining suspected cultures of the bacterium and for searching for the source of these organisms in soil, water, and other environmental niches. The reagents are highly specific for detecting the Legionnaires organism in clinical specimens.
针对嗜肺军团菌的五个菌株制备的抗血清和异硫氰酸荧光素结合物，在与来自七个地理区域患者的该生物体的10个分离株进行的同源和异源染色反应中进行了测试。根据直接免疫荧光染色结果判断，这些菌株相关但不相同。这些结合物成功用于检测福尔马林固定的肺刮片、组织切片以及活检或尸检时获得的新鲜肺组织中的嗜肺军团菌病细菌。此外，标记抗体对于对疑似细菌培养物进行染色以及在土壤、水和其他环境生态位中寻找这些生物体的来源很有价值。这些试剂在检测临床标本中的嗜肺军团菌生物体方面具有高度特异性。
