Luo Xiaomin, Zhang Boyu, Pan Yehua, Gu Jian, Tan Rui, Gong Puyang
College of Pharmacy, Southwest Minzu University, Chengdu, China.
College of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China.
Front Pharmacol. 2022 Jul 26;13:893561. doi: 10.3389/fphar.2022.893561. eCollection 2022.
Accumulating evidence suggests that dysregulation of the intestinal flora potentially contributes to the occurrence and development of nonalcoholic fatty liver disease (NAFLD). (PE), an edible and medicinal natural resource, exerts excellent effects on ameliorating NAFLD, but the potential mechanism remains unclear. In the present study, a mouse NAFLD model was established by administering a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). The protective effects of the aqueous extract of PE (AEPE) on the gut microbiota and fecal metabolites in NAFLD mice were detected by performing 16S rRNA gene sequencing and untargeted metabolomics. The administration of middle- and high-dose AEPE decreased the levels of ALT, AST, LDL-C, TG, and Hyp and increased HDL-C levels in CDAHFD-fed mice. Hematoxylin-eosin (H&E), Oil Red O, and Masson's trichrome staining indicated that AEPE treatment attenuated hepatic steatosis and fibrotic lesions. Moreover, the disordered intestinal microflora was remodeled by AEPE, including decreases in the abundance of , , and . The untargeted metabolomics analysis showed that AEPE restored the disturbed glutathione metabolism, tryptophan metabolism, taurine and hypotaurine metabolism, and primary bile acid biosynthesis of the gut bacterial community in NAFLD mice, which strongly correlated with hepatic steatosis and fibrosis. Collectively, AEPE potentially ameliorates NAFLD induced by a CDAHFD through a mechanism associated with its modulatory effects on the gut microbiota and microbial metabolism.
越来越多的证据表明，肠道菌群失调可能是非酒精性脂肪性肝病（NAFLD）发生和发展的原因。松口蘑（PE）是一种可食用和药用的天然资源，对改善NAFLD具有显著效果，但其潜在机制尚不清楚。在本研究中，通过给予胆碱缺乏、L-氨基酸定义的高脂饮食（CDAHFD）建立小鼠NAFLD模型。通过16S rRNA基因测序和非靶向代谢组学检测松口蘑水提取物（AEPE）对NAFLD小鼠肠道微生物群和粪便代谢物的保护作用。中、高剂量AEPE给药可降低CDAHFD喂养小鼠的ALT、AST、LDL-C、TG和Hyp水平，并提高HDL-C水平。苏木精-伊红（H&E）、油红O和Masson三色染色表明，AEPE治疗减轻了肝脏脂肪变性和纤维化病变。此外，AEPE重塑了紊乱的肠道微生物群，包括、和丰度的降低。非靶向代谢组学分析表明，AEPE恢复了NAFLD小鼠肠道细菌群落中受干扰的谷胱甘肽代谢、色氨酸代谢、牛磺酸和亚牛磺酸代谢以及初级胆汁酸生物合成，这与肝脏脂肪变性和纤维化密切相关。总之，AEPE可能通过与其对肠道微生物群和微生物代谢的调节作用相关的机制改善CDAHFD诱导的NAFLD。
