Li Zhengdou, Wang Xueqi, Li Xu, Chen Xia, Wang Cancan, Mao Ying, Teng Guixiang, Zhu Xinliang, Zhang Ji
College of Life Science, Northwest Normal University, Lanzhou, 730070, Gansu Province, China.
Gansu Jingbo Biotechnology Development Co., LTD, Lanzhou, 730070, Gansu Province, China.
Chem Biodivers. 2025 Jan;22(1):e202401538. doi: 10.1002/cbdv.202401538. Epub 2024 Nov 14.
Nonalcoholic fatty liver disease (NAFLD) is closely related to gut microbiota due to the hepatic portal system, and utilizing natural polysaccharides as prebiotics has become a prospective strategy for treating NAFLD. However, the therapeutic effects and potential molecular mechanisms of Lanzhou Lily polysaccharides (LLP) on NAFLD remains unclear. Therefore, the alleviating effects of LLP on NAFLD induced by high-fat diet (HFD) were investigated. LLP treatment greatly ameliorated NAFLD by significantly reducing lipid accumulation and the levels of liver function markers in HFD-induced NAFLD mice, as evidenced by decreased serum levels of TG, TC, HDL-C and LDL-C. Furthermore, LLP administration reduced hepatic steatosis, as shown by H&E and Oil red O staining. LLP also inhibited the TNF-α and IL-1β expression, thereby reducing levels of hepatic proinflammatory cytokines. Furthermore, LLP restored gut microbiota dysbiosis (up-regulated Bacteroidota, Proteobacteria, Alistipes and Lachnospiraceae abundances, down-regulated Firmicutes, Verrucomicrobiota, Desulfobacterota and Turicibacter abundances), and regulated microbial metabolic pathways such as primary bile acid biosynthesis and amino acid metabolism. In addition, the resultes of Spearman's correlation analysis found that some key metabolites in these metabolic pathways were associated with intestinal microorganisms such as Desulfobacterota, Prevotellaceae-UCG-001, Colidextribacter and Alistipes. Therefore, our study suggests that LLP may has potential applications in the treatment of NAFLD by regulating gut microbiota and its metabolite profile.
非酒精性脂肪性肝病（NAFLD）由于肝门静脉系统与肠道微生物群密切相关，利用天然多糖作为益生元已成为治疗NAFLD的一种前瞻性策略。然而，兰州百合多糖（LLP）对NAFLD的治疗效果和潜在分子机制仍不清楚。因此，研究了LLP对高脂饮食（HFD）诱导的NAFLD的缓解作用。LLP治疗通过显著减少HFD诱导的NAFLD小鼠的脂质积累和肝功能标志物水平，极大地改善了NAFLD，血清TG、TC、HDL-C和LDL-C水平降低证明了这一点。此外，H&E和油红O染色显示LLP给药减少了肝脏脂肪变性。LLP还抑制了TNF-α和IL-1β的表达，从而降低了肝脏促炎细胞因子的水平。此外，LLP恢复了肠道微生物群失调（上调拟杆菌门、变形菌门、阿里斯杆菌属和毛螺菌科的丰度，下调厚壁菌门、疣微菌门、脱硫杆菌门和Turicibacter的丰度），并调节了微生物代谢途径，如初级胆汁酸生物合成和氨基酸代谢。此外，Spearman相关性分析结果发现，这些代谢途径中的一些关键代谢物与脱硫杆菌门、普雷沃氏菌科-UCG-001、Colidextribacter和阿里斯杆菌属等肠道微生物有关。因此，我们的研究表明，LLP可能通过调节肠道微生物群及其代谢物谱在NAFLD治疗中具有潜在应用。
