Acupuncture and Tuina School/The 3rd Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Acupuncture and Chronobiology Key Laboratory of Sichuan Province, Chengdu, China.
J Gastroenterol Hepatol. 2022 Oct;37(10):1963-1974. doi: 10.1111/jgh.15981. Epub 2022 Aug 23.
We aim to investigate the effects and mechanisms of electroacupuncture (EA) at ST25 and ST37 on the intestinal low-grade inflammation (LGI) in rat model of Diarrhea-predominant irritable bowel syndrome (IBS-D).
IBS-D model rats were established by acetic acid enema combined with restraint and tail clamping. Before EA intervention, they were divided into three groups: blank 1 group, blank 2 group, and IBS-D model group. Diarrhea symptoms and visceral pain sensitivity were evaluated. After constructed the model successfully, the remaining IBS-D model group rats were randomly divided into model group and EA group. Local intestinal inflammation (HE staining), changes of intestinal mucosa (occludin protein and microvascular diameter) were evaluated. Differences between two groups were compared using t-test or Mann-Whitney U-test. Differences among more than two groups were compared using one-way ANOVA or Kruskal-Wallis test.
After modeling, the results of HE staining in intestinal tract of IBS-D model rats showed LGI. Compared with the model group, 4 h fecal moisture content (diarrhea index) and the AWR score were decreased in the EA group. The results of HE in EA group showed that the infiltration of intestinal inflammatory cells were alleviated. Additionally, EA significantly upregulated the expression of occludin protein and partially dilated the intestinal microvascular diameter. Pearson correlation analysis showed that the symptoms of IBS-D rats were correlated with the changes of intestinal mucosa.
EA may treat intestinal LGI in IBS-D rats by upregulating the expression of occludin protein and dilating the intestinal microvascular diameter.
本研究旨在探讨电针 ST25 与 ST37 对腹泻型肠易激综合征（IBS-D）大鼠模型肠道低度炎症（LGI）的作用及机制。
采用醋酸灌肠联合束缚夹尾法建立 IBS-D 模型大鼠。电针干预前，将大鼠分为空白 1 组、空白 2 组和 IBS-D 模型组。评估腹泻症状和内脏痛敏。模型成功建立后，将剩余 IBS-D 模型组大鼠随机分为模型组和电针组。评估局部肠道炎症（HE 染色）、肠黏膜变化（occludin 蛋白和微血管直径）。采用 t 检验或 Mann-Whitney U 检验比较两组间差异，采用单因素方差分析或 Kruskal-Wallis 检验比较三组以上组间差异。
造模后，IBS-D 模型大鼠肠道 HE 染色显示 LGI。与模型组相比，电针组大鼠 4 h 粪便含水量（腹泻指数）和 AWR 评分降低。电针组 HE 结果显示，肠黏膜炎症细胞浸润减轻。此外，电针可显著上调 occludin 蛋白表达，部分扩张肠微血管直径。Pearson 相关分析显示，IBS-D 大鼠症状与肠黏膜变化相关。
电针可能通过上调 occludin 蛋白表达和扩张肠微血管直径来治疗 IBS-D 大鼠的肠道 LGI。
