Ríos de Molina M C, Wainstok de Calmanovici R, San Martín de Viale L C
Int J Biochem. 1987;19(4):365-72. doi: 10.1016/0020-711x(87)90010-3.
The present work studies the action of hexachlorobenzene (HCB) on the decarboxylation of uroporphyrinogen (Urogen) I and III and also on the decarboxylation of intermediate porphyrinogens of series III under different conditions using liver of normal and porphyric rats as enzyme source. The same enzyme is involved in the Urogen decarboxylation of both isomeric series I and III and catalyses the four steps in both cases. HCB affects all of them. HCB blocks the four steps of Urogen III decarboxylation to the same degree, as a function of intoxication time. HCB leads, in general, to an increase in the efficiency (Km/Vmax) of the porphyric system. These data can be interpreted as a reaction of the organism to overcome the enzymatic blockade.
本研究以正常大鼠和卟啉症大鼠的肝脏为酶源，研究了六氯苯（HCB）在不同条件下对尿卟啉原（Urogen）I和III脱羧作用的影响，以及对III系列中间卟啉原脱羧作用的影响。参与I和III两种异构体系列Urogen脱羧作用的是同一种酶，且在两种情况下都催化四个步骤。HCB对所有这些步骤都有影响。HCB根据中毒时间的长短，同等程度地阻断Urogen III脱羧的四个步骤。总体而言，HCB导致卟啉系统的效率（Km/Vmax）提高。这些数据可以解释为机体克服酶阻断的一种反应。
