CircSHOC2基因敲低通过靶向miR-145/FOXO1轴在体外条件下减轻高糖诱导的血管内皮细胞焦亡。
CircSHOC2 Knockdown Alleviates High Glucose-Induced Vascular Endothelial Cell Pyroptosis via Targeting miR-145/FOXO1 Axis In Vitro Condition.
作者信息
Meng Xuying, Li Zhenjin, Li Yang
机构信息
Department of Endocrinology, The Second Hospital of Tianjin Medical University, Tianjin, China.
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China.
出版信息
Mol Biotechnol. 2023 Mar;65(3):384-393. doi: 10.1007/s12033-022-00544-7. Epub 2022 Aug 12.
Emerging evidence indicates that pyroptosis participates in the pathogenic process of vascular endothelial cells in cardiovascular system complications of diabetes. The roles of circular RNAs (circRNAs) in high glucose (HG)-induced vascular endothelial cells are still unclear. Here, our research investigated the function and mechanism of circRNA circSHOC2 in pyroptosis of vascular endothelial cells. Results indicated that circSHOC2 was up-regulated in HG-induced human umbilical vein endothelial cells (HUVECs). Functionally, cellular assays indicated that circSHOC2 silencing repressed HG-induced HUVECs pyroptosis. Moreover, circSHOC2 targeted miR-145 through miRNA sponge, and FOXO1 functioned as downstream target of miR-145. In conclusion, these findings suggested the potential roles of circSHOC2 on HG-induced vascular endothelial cells in vitro condition, providing new insights for cardiovascular system complications of diabetes.
新出现的证据表明，细胞焦亡参与了糖尿病心血管系统并发症中血管内皮细胞的致病过程。环状RNA（circRNA）在高糖（HG）诱导的血管内皮细胞中的作用仍不清楚。在此，我们的研究探讨了circRNA circSHOC2在血管内皮细胞焦亡中的功能和机制。结果表明，circSHOC2在HG诱导的人脐静脉内皮细胞（HUVECs）中上调。在功能上，细胞实验表明circSHOC2沉默可抑制HG诱导的HUVECs焦亡。此外，circSHOC2通过miRNA海绵靶向miR-145，而FOXO1作为miR-145的下游靶点发挥作用。总之，这些发现提示了circSHOC2在体外条件下对HG诱导的血管内皮细胞的潜在作用，为糖尿病心血管系统并发症提供了新的见解。
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