Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
Cold Spring Harb Protoc. 2022 Oct 3;2022(10):Pdb.prot107990. doi: 10.1101/pdb.prot107990.
Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity of the salivary glands must be closely monitored throughout the entire process to prevent structural damage and loss of saliva. This protocol describes a series of simple steps to perform salivary gland immunohistochemistry including tissue dissection, permeabilization, immunostaining, mounting, and imaging by confocal microscopy.
免疫组织化学是一种有价值的技术，可提供组织中蛋白质定位和相互作用的信息。蚊子唾液腺免疫组织化学需要对精细组织进行精心解剖。在整个过程中必须密切监测唾液腺的完整性，以防止结构损伤和唾液流失。本方案描述了一系列简单的步骤来进行唾液腺免疫组织化学，包括组织解剖、通透化、免疫染色、装片和共聚焦显微镜成像。
